Detailed information of TA system TA202927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 199660..200420
• Replicon: chromosome
• Accession: NZ_CP075113
• Organism: Salmonella enterica subsp. enterica serovar Dublin strain CFSAN059898

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q57IH1
• Locus tag: CCO47_RS00935
• Protein ID: WP_000533909.1
• Coordinates: 199935..200420 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: M7RHS4
• Locus tag: CCO47_RS00930
• Protein ID: WP_000965886.1
• Coordinates: 199660..199947 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCO47_RS00910 (195065)	195065..195976	+	912	WP_001168551.1	glycine--tRNA ligase subunit alpha	-
CCO47_RS00915 (195986)	195986..198055	+	2070	WP_001291741.1	glycine--tRNA ligase subunit beta	-
CCO47_RS24045 (198445)	198445..198843	+	399	Protein_182	IS3 family transposase	-
CCO47_RS00925 (199015)	199015..199482	+	468	WP_000702452.1	GNAT family N-acetyltransferase	-
CCO47_RS00930 (199660)	199660..199947	+	288	WP_000965886.1	DUF1778 domain-containing protein	Antitoxin
CCO47_RS00935 (199935)	199935..200420	+	486	WP_000533909.1	GNAT family N-acetyltransferase	Toxin
CCO47_RS00940 (200791)	200791..201330	-	540	WP_000047147.1	copper-binding periplasmic metallochaperone CueP	-
CCO47_RS00945 (201504)	201504..201716	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
CCO47_RS00950 (202004)	202004..202294	-	291	WP_000455790.1	HTH-type transcriptional regulator	-
CCO47_RS00955 (202733)	202733..203443	+	711	WP_000190524.1	DUF3053 domain-containing protein	-
CCO47_RS00960 (203493)	203493..204467	-	975	WP_000804678.1	glyoxylate/hydroxypyruvate reductase GhrB	-
CCO47_RS00965 (204686)	204686..205348	-	663	WP_000747548.1	OmpA family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17703.41 Da
• Isoelectric Point: 9.8719

>T202927 WP_000533909.1 NZ_CP075113:199935-200420 [Salmonella enterica subsp. enterica serovar Dublin]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKSSQTQQRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T202927 NZ_CP099752:3266682-3266912 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10950.60 Da
• Isoelectric Point: 4.6811

>AT202927 WP_000965886.1 NZ_CP075113:199660-199947 [Salmonella enterica subsp. enterica serovar Dublin]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILETACQAAEKVILDRRVFNFNDEQYEEFINLLDAPVADD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT202927 NZ_CP099752:3266909-3267268 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• PDB: 7F36
• PDB: 7AK8
• PDB: 5FVJ

Antitoxin

• AlphaFold DB: A0A3V2JDX2