Detailed information of TA system TA202821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4301141..4301922
• Replicon: chromosome
• Accession: NZ_CP075106
• Organism: Salmonella enterica strain CFSAN064276

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A5I5SY00
• Locus tag: CBI76_RS20930
• Protein ID: WP_000626104.1
• Coordinates: 4301141..4301632 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B5R979
• Locus tag: CBI76_RS20935
• Protein ID: WP_001110452.1
• Coordinates: 4301629..4301922 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBI76_RS20905 (4296281)	4296281..4298719	-	2439	WP_001014138.1	F4 (K88) fimbrial usher FaeD	-
CBI76_RS20910 (4298729)	4298729..4299268	-	540	WP_000721295.1	type 1 fimbrial protein	-
CBI76_RS20915 (4299303)	4299303..4299587	-	285	WP_205903706.1	PapB/FocB family fimbrial expression transcriptional regulator	-
CBI76_RS20920 (4300176)	4300176..4300403	+	228	WP_001519955.1	hypothetical protein	-
CBI76_RS20925 (4300678)	4300678..4300926	-	249	Protein_4114	IS481 family transposase	-
CBI76_RS20930 (4301141)	4301141..4301632	-	492	WP_000626104.1	GNAT family N-acetyltransferase	Toxin
CBI76_RS20935 (4301629)	4301629..4301922	-	294	WP_001110452.1	DUF1778 domain-containing protein	Antitoxin
CBI76_RS20940 (4302239)	4302239..4302460	+	222	WP_021001044.1	hypothetical protein	-
CBI76_RS20945 (4302726)	4302726..4303601	+	876	WP_000921676.1	AraC family transcriptional regulator	-
CBI76_RS20950 (4303598)	4303598..4303885	+	288	WP_001269916.1	transcriptional regulator RtsB	-
CBI76_RS20955 (4303908)	4303908..4304123	+	216	WP_001595136.1	hypothetical protein	-
CBI76_RS20960 (4304131)	4304131..4304400	+	270	WP_010989096.1	hypothetical protein	-
CBI76_RS20965 (4304694)	4304694..4305599	-	906	WP_001268200.1	YjiK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	faeI / faeH / faeF / faeE / faeD / faeC	4289505..4304400	14895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17631.42 Da
• Isoelectric Point: 7.7297

>T202821 WP_000626104.1 NZ_CP075106:c4301632-4301141 [Salmonella enterica]
MISTPEPLHAGHILTPFCCGVDSIDNWLKQRAMKNQTTGASRTFVCCGSDSNVLAYYSLASSAVTTNTSPGRFRRNMPDP
IPVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYQRVGFVPSPMDPMMLMVTLGDLV
ESV

Nucleotide

• Length: 492 bp

>T202821 NZ_CP099705:316877-317245 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10977.61 Da
• Isoelectric Point: 8.6141

>AT202821 WP_001110452.1 NZ_CP075106:c4301922-4301629 [Salmonella enterica]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLVRLDQTPSPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT202821 NZ_CP099705:316659-316880 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A5I5SY00

Antitoxin

• PDB: 7AK7
• AlphaFold DB: A0A5I1DGA4