Detailed information of TA system TA020280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1595546..1596342
• Replicon: chromosome
• Accession: NC_002488
• Organism: Xylella fastidiosa 9a5c

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: Q9PCU8
• Locus tag: XF_RS07040
• Protein ID: WP_010894152.1
• Coordinates: 1595731..1596342 (+)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: Q9PCU9
• Locus tag: XF_RS07035
• Protein ID: WP_010894151.1
• Coordinates: 1595546..1595734 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XF_RS06995 (XF1648)	1591171..1592097	-	927	WP_010894143.1	phage recombination protein Bet	-
XF_RS07000 (XF1649)	1592113..1592934	-	822	WP_010894144.1	YfdQ family protein	-
XF_RS07005 (XF1650)	1592955..1593329	-	375	WP_010894145.1	hypothetical protein	-
XF_RS07010 (XF1651)	1593350..1593541	-	192	WP_010894146.1	hypothetical protein	-
XF_RS07015 (XF1652)	1593565..1593756	-	192	WP_010894147.1	hypothetical protein	-
XF_RS07020 (XF1653)	1593753..1594154	-	402	WP_010894148.1	hypothetical protein	-
XF_RS07025 (XF1654)	1594154..1594612	-	459	WP_010894149.1	hypothetical protein	-
XF_RS07030 (XF1655)	1594609..1595154	-	546	WP_080507209.1	hypothetical protein	-
XF_RS07035 (XF1656)	1595546..1595734	+	189	WP_010894151.1	antitoxin VbhA family protein	Antitoxin
XF_RS07040 (XF1657)	1595731..1596342	+	612	WP_010894152.1	Fic/DOC family protein	Toxin
XF_RS07045 (XF1658)	1596393..1597067	-	675	WP_042463200.1	helix-turn-helix transcriptional regulator	-
XF_RS07050	1597152..1597397	+	246	WP_042463203.1	hypothetical protein	-
XF_RS07055	1597394..1597699	-	306	WP_042463205.1	hypothetical protein	-
XF_RS07060	1597840..1598025	+	186	Protein_1376	hypothetical protein	-
XF_RS07065 (XF1660)	1598079..1598267	+	189	WP_042463208.1	hypothetical protein	-
XF_RS07070 (XF1661)	1598264..1598512	+	249	WP_010894156.1	hypothetical protein	-
XF_RS07075 (XF1662)	1598509..1598892	+	384	WP_042463212.1	hypothetical protein	-
XF_RS07080 (XF1663)	1599096..1600208	+	1113	WP_042463693.1	Bro-N domain-containing protein	-
XF_RS07085 (XF1664)	1600205..1600513	+	309	WP_023906549.1	DUF1376 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22892.65 Da
• Isoelectric Point: 4.9498

>T20280 WP_010894152.1 NC_002488:1595731-1596342 [Xylella fastidiosa 9a5c]
MKYAGDRGDPYLDSETGVLRNLLGISDQGWLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T20280 NC_002488:1595731-1596342 [Xylella fastidiosa 9a5c]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGCGA
TCAGGGGTGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGCCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCGATTTTTGTGTGGCCTTGATGCTGAGGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TGCTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAACGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACCGG
ATCGACTGGGGCGTGGTCAGCCAGGCGGATATGATTAAAGCGTCAATCGACGCTTACAACGGGGATTCAAGCGGATTGGC
TAGTATCATTCGCGAAGGAATATCTGATCAGCTTTTTAAAGATAATGAGTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6885.83 Da
• Isoelectric Point: 4.9859

>AT20280 WP_010894151.1 NC_002488:1595546-1595734 [Xylella fastidiosa 9a5c]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIATAVDQYKARVRLQMA

Nucleotide

• Length: 189 bp

>AT20280 NC_002488:1595546-1595734 [Xylella fastidiosa 9a5c]
ATGCTGACAGATCATGAGAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAGGCCGTGGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTACCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATGGCATGA

Structures

Toxin

• AlphaFold DB: Q9PCU8

Antitoxin

• AlphaFold DB: Q9PCU9