Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14187..14830 Replicon plasmid pCTX_J9_3
Accession NZ_CP075064
Organism Escherichia coli strain EC_J_9

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag H4F96_RS24665 Protein ID WP_001044768.1
Coordinates 14414..14830 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag H4F96_RS24660 Protein ID WP_001261287.1
Coordinates 14187..14417 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F96_RS24640 (9347) 9347..10435 + 1089 WP_000952217.1 transcriptional repressor PifC -
H4F96_RS24645 (10437) 10437..12662 + 2226 WP_000698737.1 P-loop NTPase fold protein -
H4F96_RS24650 (12712) 12712..13611 - 900 WP_000963206.1 nucleotide-binding protein -
H4F96_RS24655 (13601) 13601..13891 - 291 WP_000111771.1 hypothetical protein -
H4F96_RS24660 (14187) 14187..14417 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H4F96_RS24665 (14414) 14414..14830 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
H4F96_RS24670 (14992) 14992..17130 - 2139 WP_000350638.1 AAA family ATPase -
H4F96_RS24675 (17484) 17484..17741 + 258 WP_000343085.1 hypothetical protein -
H4F96_RS24680 (17741) 17741..18331 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / blaTEM-1B / blaCTX-M-55 - 1..101266 101266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T202794 WP_001044768.1 NZ_CP075064:14414-14830 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T202794 NZ_CP099610:4810238-4810492 [Pseudomonas moraviensis]
ATGCAAACCATCAACTACACCACCGCCCGTGCGCATTTGGCCGAGACGATGGACCGTGTCAATGAAGATCGTGCGCCGCT
ATTGGTCACTCGGCAGAAAGGTGAACCAGTAGTGATGATGTCTTTGGCCGAATACAACGCATTGGAAGAAACAGCGTATT
TGCTTCGCTCCCCCGCCAACGCCGAACGGCTGATGAAATCCATCGGTGAGTTACGCGCCGGCAAAGCCAAAGTCAGGCAA
TTGATCGAAGAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT202794 WP_001261287.1 NZ_CP075064:14187-14417 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT202794 NZ_CP099610:4810489-4810743 [Pseudomonas moraviensis]
ATGAAAATCGAGTTCACGCCCACAGGCTGGGAAGACTATTTGTGGTTTCAGCAAAACGACAAGGCTGGGCTCAAGCGCAT
CAATCTTCTGATCAAAGCAATCAAGCGCGAACCGTTTGAGGGTGTCGGTAAACCAGAGGCGCTCAAACATAATATGAGCG
GCCTCTGGTCACGCCGAGTAACGGCCGAACATCGTCTGGTGTACAGGGTTGAGGACGGCGAAATCTGCGTCATCAGTTGC
CGGTATCACTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References