Detailed information of TA system TA202790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 85536..86161
• Replicon: plasmid pOQX_J9_2
• Accession: NZ_CP075063
• Organism: Escherichia coli strain EC_J_9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H4F96_RS24235
• Protein ID: WP_000911313.1
• Coordinates: 85763..86161 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: H4F96_RS24230
• Protein ID: WP_000450520.1
• Coordinates: 85536..85763 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F96_RS24230 (85536)	85536..85763	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H4F96_RS24235 (85763)	85763..86161	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
H4F96_RS24240 (86170)	86170..88323	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
H4F96_RS24245 (88576)	88576..89307	-	732	WP_021499552.1	conjugal transfer complement resistance protein TraT	-
H4F96_RS24250 (89339)	89339..89836	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..140375	140375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T202790 WP_000911313.1 NZ_CP075063:85763-86161 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T202790 NZ_CP099610:c2244546-2244238 [Pseudomonas moraviensis]
ATGAGCCAGACTTTGACGAATTTCGACATGGTCGCGCTGCTCGACAGTGATGAAGCCATCAGTGAGTACCTGTCACAGGT
GCTTGCAGACGGAGACAGCGAAGAATTCCTTCGCGCAATCGGTTACGTTTTAAGAGCGCGAGGAATGACGCAGATCGCCA
AGAATTCAGGCATGGGTCGTGAAAGTTTGTACAAAGCTTTTGCTCCCGGAGCGAAGCCGCGCTTTGACACAGTGTTGAAG
GTGATTCATGCGTTGGGCATTGATCTTTATGCGCAGCCGGGGCACGTAGTCAATCACGCCGCTACCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT202790 WP_000450520.1 NZ_CP075063:85536-85763 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT202790 NZ_CP099610:c2244842-2244549 [Pseudomonas moraviensis]
ATGACTTATCTTATCCAGCAAACCGCAATATTCCACGCTTGGCACCGTTCGATTAGAGACTTGCGCGCCAAAGTGGCTAT
CGCCCGGCGTATTGATCGGGCGTCAACAGGTAATCTCGGCGACGCCAAATCGGTGGGCGATGGAGTCTCTGAGATGCGTC
TGGATGTTGGCGCGGGCTACCGGGTGTATTTCACAATCCGCAATGGCGCAGTAATCATTCTGCTTGCCGGAGGCGACAAA
TCCTCACAAAGCGCAGACATCCGACGCGCACAAAAAATGGCAAAGGAGGTTTAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2