Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4454600..4455097 Replicon chromosome
Accession NZ_CP075062
Organism Escherichia coli strain EC_J_9

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H4F96_RS21530 Protein ID WP_001173343.1
Coordinates 4454600..4454773 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H4F96_RS21535 Protein ID WP_000398619.1
Coordinates 4454801..4455097 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F96_RS21520 (4451270) 4451270..4452727 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
H4F96_RS21525 (4452964) 4452964..4454481 + 1518 WP_001295087.1 lysine--tRNA ligase -
H4F96_RS21530 (4454600) 4454600..4454773 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H4F96_RS21535 (4454801) 4454801..4455097 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H4F96_RS21540 (4455324) 4455324..4455596 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
H4F96_RS21545 (4455608) 4455608..4455838 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
H4F96_RS21550 (4456019) 4456019..4457650 + 1632 WP_001216487.1 sensor histidine kinase -
H4F96_RS21555 (4457647) 4457647..4458366 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T202784 WP_001173343.1 NZ_CP075062:c4454773-4454600 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T202784 NZ_CP099609:4869288-4869548 [Pseudomonas moraviensis]
ATGAAAATCGAGTTCACGCCCACAGGCTGGGAAGACTATTTGTGGTTTCAGCAAAACGACAAGGCTGGGCTCAAGCGCAT
CAATCTTCTGATCAAAGCAATCAAGCGCGAACCGTTTGAGGGTGTCGGTAAACCAGAGGCGCTCAAGCATAATATGAGCG
GCCTCTGGTCACGCCGAGTAACGGCCGAACATCGTCTGGTGTACAGGGTTGAGGACGGCGAAATCTGCGTCATCAGTTGC
CGGTATCACTATTCAACCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT202784 WP_000398619.1 NZ_CP075062:c4455097-4454801 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT202784 NZ_CP099609:4869037-4869291 [Pseudomonas moraviensis]
ATGCAAACCATCAACTACACCACCGCCCGTGCGCATTTGGCCGAGACGATGGACCGTGTCAATGAAGATCGTGCGCCGCT
ATTGGTCACTCGGCAGAAAGGTGAACCAGTAGTGATGATGTCTTTGGCCGAATACAACGCATTGGAAGAAACAGCGTACT
TGCTTCGCTCCCCCGCCAACGCCGAACGGCTGATGAAATCCATCGGTGAGTTACGCGCCGGCAAAGCCAAAGTCAGGCAA
TTGATCGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References