Detailed information of TA system TA202782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3710437..3711274
• Replicon: chromosome
• Accession: NZ_CP075062
• Organism: Escherichia coli strain EC_J_9

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: H4F96_RS18065
• Protein ID: WP_000227784.1
• Coordinates: 3710732..3711274 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: H4F96_RS18060
• Protein ID: WP_001297137.1
• Coordinates: 3710437..3710748 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F96_RS18035 (3705457)	3705457..3706404	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
H4F96_RS18040 (3706426)	3706426..3708417	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
H4F96_RS18045 (3708407)	3708407..3709021	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
H4F96_RS18050 (3709021)	3709021..3709350	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
H4F96_RS18055 (3709362)	3709362..3710252	+	891	WP_000971336.1	heme o synthase	-
H4F96_RS18060 (3710437)	3710437..3710748	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
H4F96_RS18065 (3710732)	3710732..3711274	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
H4F96_RS18070 (3711330)	3711330..3712265	-	936	WP_001368479.1	tetratricopeptide repeat protein	-
H4F96_RS18075 (3712673)	3712673..3714037	+	1365	WP_001000960.1	MFS transporter	-
H4F96_RS18080 (3714165)	3714165..3714656	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
H4F96_RS18085 (3714824)	3714824..3715735	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T202782 WP_000227784.1 NZ_CP075062:3710732-3711274 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T202782 NZ_CP099609:3416853-3417047 [Pseudomonas moraviensis]
ATTCCGACGCAAAGCGGGAGCCGCAGCGCAAAACTTTCTTCCATCCCCTACGGCTACCAATCCGGTCGGGTGCCTGGCAC
TCGCGAAATGGTGATCCATCCTAACTATCTTCTGGTTTATCGGGTGAACGGGGGTATCCAAATTCTGAGGGTACTTCACG
CTCGACAAAAATATCCCCGAACCCATTCACTCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT202782 WP_001297137.1 NZ_CP075062:3710437-3710748 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT202782 NZ_CP099609:3416580-3416774 [Pseudomonas moraviensis]
ATGAGCGCTGAACTTTCACCTATCGTTTCAGACTTCGAAACCGAAGAAGAGGCTGCGGATTACGACCTTTGGTTTCGTGC
GCAGGTCCAGGAAGCACTTGATGATCCGCATCCGGGCGTTCCCCATGCGGAAGCCATGGTGATGCTCGACCAGATGATTG
AAAAAAACCGCAGGAAGCGCCGCGCTGCAGGTTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3