Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 958593..959247 Replicon chromosome
Accession NZ_CP075062
Organism Escherichia coli strain EC_J_9

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag H4F96_RS04685 Protein ID WP_063112724.1
Coordinates 958840..959247 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H4F96_RS04680 Protein ID WP_000354046.1
Coordinates 958593..958859 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F96_RS04655 (953881) 953881..954624 + 744 WP_000951964.1 SDR family oxidoreductase -
H4F96_RS04660 (954681) 954681..956114 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
H4F96_RS04665 (956159) 956159..956470 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
H4F96_RS04670 (956634) 956634..957293 + 660 WP_000250274.1 hemolysin III family protein -
H4F96_RS04675 (957370) 957370..958350 - 981 WP_001472148.1 tRNA-modifying protein YgfZ -
H4F96_RS04680 (958593) 958593..958859 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H4F96_RS04685 (958840) 958840..959247 + 408 WP_063112724.1 protein YgfX Toxin
H4F96_RS04690 (959287) 959287..959808 - 522 WP_001055874.1 flavodoxin FldB -
H4F96_RS04695 (959920) 959920..960816 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H4F96_RS04700 (960841) 960841..961551 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H4F96_RS04705 (961557) 961557..963290 + 1734 WP_000813210.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16082.04 Da        Isoelectric Point: 11.2511

>T202766 WP_063112724.1 NZ_CP075062:958840-959247 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLFHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T202766 NZ_CP099602:c4793813-4793469 [Pseudomonas siliginis]
ATGCGAACAATCTTTTTTGAAACAACGATATTTACCAAAAGCGTTGGCCGCTATCTGACGGACGACGAATATCGAACATT
GCAGAACTACCTTCAGAGCAATCCGCTTGCGGGCGATGTAATGCCTCGAACGGGTGGCTTCCGTAAGCTGCGCTGGGCTG
ATTCTCGCCGTAGCAAGGGAAGACGGGGCGGCTTGCGGGTTCTCTACTATTGGCTTATGAATGATGGTCAGTTCTGGATG
TTCGCCATTTACGACAAAGATGAGCTTGAGAAGCTGACTGCCGAGCAAGAGAGGGCACTCAGGACAGCCATTGATAAAGA
GTTGAAAAAACGAGGTACCCCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT202766 WP_000354046.1 NZ_CP075062:958593-958859 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT202766 NZ_CP099602:c4793472-4793158 [Pseudomonas siliginis]
ATGAAAAAACGCGACCTGTTTGCAGAGTTAATGCAGGGCGTTGATGAGATGGCGGCTCAACGCGAGGGCAAGATCACACT
GCGCAATACAGTGCTTGAGGACAAGCCTGTACCTGAAGTGGGCGCACAGGAAATTGTTGCTCTTCGAGAAAAACTGCATA
TGTCTCAAGCTGTTTTTGCCAGTCGAATCAGGACAAGTCCAAACACCCTTCGAAATTGGGAGCAGGAAAAGTCGAAGCCC
AATGCTCAAGCAGCCTTATTGATACGACTGGTTGAAAAATTTCCAGATATGGTTGAGCGCTTGAACACTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References