Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 819127..819925 Replicon chromosome
Accession NZ_CP075062
Organism Escherichia coli strain EC_J_9

Toxin (Protein)


Gene name yeeV Uniprot ID U9XMP3
Locus tag H4F96_RS03950 Protein ID WP_000854735.1
Coordinates 819127..819504 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M1EW42
Locus tag H4F96_RS03955 Protein ID WP_032153712.1
Coordinates 819551..819925 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F96_RS03920 (815095) 815095..816357 - 1263 WP_001218810.1 integrase arm-type DNA-binding domain-containing protein -
H4F96_RS03925 (816821) 816821..817147 - 327 WP_000779483.1 type II toxin-antitoxin system RelE/ParE family toxin -
H4F96_RS03930 (817144) 817144..817407 - 264 WP_025492088.1 type II toxin-antitoxin system ParD family antitoxin -
H4F96_RS03935 (817479) 817479..818345 - 867 WP_032153714.1 DUF4942 domain-containing protein -
H4F96_RS03940 (818430) 818430..818627 - 198 WP_000839265.1 DUF957 domain-containing protein -
H4F96_RS03945 (818639) 818639..819130 - 492 WP_023155722.1 DUF5983 family protein -
H4F96_RS03950 (819127) 819127..819504 - 378 WP_000854735.1 TA system toxin CbtA family protein Toxin
H4F96_RS03955 (819551) 819551..819925 - 375 WP_032153712.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
H4F96_RS03960 (820005) 820005..820226 - 222 WP_000692345.1 DUF987 domain-containing protein -
H4F96_RS03965 (820295) 820295..820771 - 477 WP_001186715.1 RadC family protein -
H4F96_RS03970 (820787) 820787..821272 - 486 WP_032153711.1 antirestriction protein -
H4F96_RS03975 (821364) 821364..822182 - 819 WP_001234693.1 DUF932 domain-containing protein -
H4F96_RS03980 (822273) 822273..822482 - 210 WP_023155727.1 DUF905 family protein -
H4F96_RS03985 (822512) 822512..823189 - 678 WP_023155728.1 hypothetical protein -
H4F96_RS03990 (823308) 823308..824192 - 885 WP_024166630.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM 739906..840061 100155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14061.07 Da        Isoelectric Point: 8.2904

>T202765 WP_000854735.1 NZ_CP075062:c819504-819127 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T202765 NZ_CP099602:3290937-3291158 [Pseudomonas siliginis]
ATCGAATACGTAGAACAACGCAACACTGTCGCTTCAGTTGCACTGCACCAAAGGCTCAGCGCCGCTGCGCGAAGGCTTTC
CTGGATACCGCATGGTTTCCGCTCCGGCCGAATACCGGGCACTCGCGAAATGGTCATCAACTCGAATTATTTACTGGTCT
ATCAGGTGACAGACCACATCAAAATTCTGACCGTGCTGCACGCCCGAAAAAAATATCCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13697.51 Da        Isoelectric Point: 6.6240

>AT202765 WP_032153712.1 NZ_CP075062:c819925-819551 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT202765 NZ_CP099602:3290705-3290899 [Pseudomonas siliginis]
ATGAGCGCTGAACTTTCACTTATCGTTTCAGACTTCGAAACCGAAGAAGAGGCTGCGGATTACGATCTTTGGTATCGCGC
CAAGGTACAAGCCGCACTTGATGATCCACGTCCGCCTATCCCCCACGATGAGGCAATGGTGCTGCTAGGTCAGATGCTGG
AAGAGAGACGCAAGAATCGACGCGCTGCCGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XMP3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1EW42

References