Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 267289..267920 Replicon chromosome
Accession NZ_CP075062
Organism Escherichia coli strain EC_J_9

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag H4F96_RS01190 Protein ID WP_001259388.1
Coordinates 267289..267564 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H4F96_RS01195 Protein ID WP_000593555.1
Coordinates 267561..267920 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F96_RS01175 (262293) 262293..263360 + 1068 WP_000361485.1 HlyD family secretion protein -
H4F96_RS01180 (263357) 263357..266092 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
H4F96_RS01185 (266092) 266092..267216 + 1125 WP_001314210.1 ABC transporter permease -
H4F96_RS01190 (267289) 267289..267564 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
H4F96_RS01195 (267561) 267561..267920 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H4F96_RS01200 (268040) 268040..268441 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H4F96_RS01205 (268447) 268447..269253 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H4F96_RS01210 (269250) 269250..270014 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H4F96_RS01215 (270014) 270014..270847 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
H4F96_RS01220 (270844) 270844..271788 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 259690..267920 8230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T202762 WP_001259388.1 NZ_CP075062:267289-267564 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T202762 NZ_CP099602:1786462-1786674 [Pseudomonas siliginis]
GTGCAGAGCAGGCTATTGATGAAGGAGCTGGAGGAGGCGGGCTGGACGCTGGATCGGGTTGCGGGCAGTCATCACATCTT
CACTCATCGTTACAACCCGAACACGATTGCCGTCCCGCATCCGAAAAAGGATTTACCGTTGGGGACAGTCAAAAGCATTC
GGCGGCGCGCGGGGTTATTCAGCCCGCCAACCCGTCATGAAGGAGATCCGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT202762 WP_000593555.1 NZ_CP075062:267561-267920 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT202762 NZ_CP099602:1786674-1787078 [Pseudomonas siliginis]
ATGCAATATCCCATCTGCATCGAGTGGGGTGACGAGAACACTGCCATCGGCATTCAGATCCCCGATATTCCCGGCGCAGT
CACGGCCGGGGACAGCTTTGAGGAGGCCTACAACGCGGCCGTGGAAGTGGCTCACCTCATGCTGCAGGAGATCGCGGCGG
CCGGTCAGGCGATTCCGATGCCGACCTCGGCAGCGGCGCATCGCAATCATCCGGATTTTGCCGAGATGGGCTGGGGCATG
CTGGAGCTGGATATCTCGCCGTACCTGGGCAAGACCGAAAAGGTCAACGTTACGCTGCCCGGCTATGTCATTCAGCGTAT
TGATCGCTATGTACGCGAGCATAACGTCAAAAGCCGCTCCTCGTTTCTGGCGGATGCGGCGATGGAGAAACTGGTTCGGT
ATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References