Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3683080..3683663 Replicon chromosome
Accession NZ_CP075061
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag H4F95_RS20805 Protein ID WP_000254745.1
Coordinates 3683080..3683415 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag H4F95_RS20810 Protein ID WP_000581937.1
Coordinates 3683415..3683663 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS20785 (3678549) 3678549..3678911 + 363 WP_000034928.1 hypothetical protein -
H4F95_RS20790 (3678967) 3678967..3680265 - 1299 WP_000036723.1 phosphopyruvate hydratase -
H4F95_RS20795 (3680353) 3680353..3681990 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
H4F95_RS20800 (3682218) 3682218..3683009 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
H4F95_RS20805 (3683080) 3683080..3683415 - 336 WP_000254745.1 endoribonuclease MazF Toxin
H4F95_RS20810 (3683415) 3683415..3683663 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
H4F95_RS20815 (3683741) 3683741..3685975 - 2235 WP_000226815.1 GTP pyrophosphokinase -
H4F95_RS20820 (3686023) 3686023..3687324 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T202758 WP_000254745.1 NZ_CP075061:c3683415-3683080 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T202758 NZ_CP099602:174303-174584 [Pseudomonas siliginis]
ATGACTTATAAGCTTGAGTTTCTGCCGTCTGCACACAAAGAGTGGAACAAGCTGGGTCATACATTGCGTGAGCAATTCAA
AAAGAAGCTAGGTGAGAGGCTGAAGCTTCCCAGAATCCCGGCTGACGCACTCCACGGCATGCCCGACTGCTACAAAATCA
AACTCAAAGCCTCGGGGTATCGATTGGTTTACCAAGTCATCGACGAACGAGTCGTGGTTTCCGTGCTAGCGGTTGGCAAG
CGCGAACGCAGCAGCGTTTACGATGCTGCCAGGAACCGTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT202758 WP_000581937.1 NZ_CP075061:c3683663-3683415 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT202758 NZ_CP099602:174068-174313 [Pseudomonas siliginis]
ATGCAAAGCGTTCTCGCTGATATGGCTGTCAGTGTCTCTGAACTGAAGAAAAATCCTACTGCCGTGTTGAACGGTGCACA
TGGGGGGGCCGTCGCTGTCCTCAACCACAATCGCGTCATGGGCTATATGGTGCCGGCCGAAGTTTACGAAGCGATGATGG
AGCGGCTTGATGATCTTGAACTGGCTGACATTGTTCGTTCGCGTCTCCACGAAACGCCCATTTCGGTAAGCCTGGATGAC
TTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References