Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3362909..3363534 Replicon chromosome
Accession NZ_CP075061
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag H4F95_RS19175 Protein ID WP_000911329.1
Coordinates 3362909..3363307 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag H4F95_RS19180 Protein ID WP_000450524.1
Coordinates 3363307..3363534 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS19155 (3358787) 3358787..3358987 + 201 WP_000383836.1 YpfN family protein -
H4F95_RS19160 (3359097) 3359097..3359795 - 699 WP_000679812.1 esterase -
H4F95_RS19165 (3359869) 3359869..3361884 - 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
H4F95_RS19170 (3361899) 3361899..3362762 - 864 WP_001267507.1 neutral zinc metallopeptidase -
H4F95_RS19175 (3362909) 3362909..3363307 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
H4F95_RS19180 (3363307) 3363307..3363534 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
H4F95_RS19185 (3363690) 3363690..3364403 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
H4F95_RS19190 (3364616) 3364616..3365650 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
H4F95_RS19195 (3365667) 3365667..3366545 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
H4F95_RS19200 (3366691) 3366691..3367263 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
H4F95_RS19205 (3367263) 3367263..3367733 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T202757 WP_000911329.1 NZ_CP075061:c3363307-3362909 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T202757 NZ_CP099601:c4794746-4794402 [Pseudomonas siliginis]
ATGCGAACAATCTTTTTTGAAACAACGATATTTACCAAAAGCGTTGACCGCTATCTGACGGACGACGAATATCGAACATT
GCAGAACTACCTTCAGAGCAATCCGCTTGCGGGCGATGTAATGCCTCGAACGGGTGGCTTCCGTAAGCTGCGCTGGGCTG
ATTCTCGCCGTAGCAAGGGAAGACGGGGCGGCTTGCGGGTTCTCTACTATTGGCTTATGAATGATGGTCAGTTCTGGATG
TTCGCCATTTACGACAAAGATGAGCTTGAGAAGCTGACTGCCGAGCAAGAGAGGGCACTCAGGACAGCCATTGATAAAGA
GTTGAAAAAACGAGGTACCCCATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT202757 WP_000450524.1 NZ_CP075061:c3363534-3363307 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT202757 NZ_CP099601:c4794405-4794091 [Pseudomonas siliginis]
ATGAAAAAACGCGACCTGTTTGCAGAGTTAATGCAGGGCGTTGATGAGATGGCGGCTCAACGCGAGGGCAAGATCACACT
GCGCAATACAGTGCTTGAGGACAAGCCTGTACCTGAAGTGGGCGCACAGGAAATTGTTGCTCTTCGAGAAAAACTGCATA
TGTCTCAAGCTGTTTTTGCCAGTCGAATCAGGACAAGTCCAAACACCCTTCGAAATTGGGAGCAGGAAAAGTCGAAGCCC
AATGCTCAAGCAGCCTTATTGATACGACTGGTTGAAAAATTTCCAGATATGGTTGAGCGCTTGAACACTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References