Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2251064..2251702 Replicon chromosome
Accession NZ_CP075061
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H4F95_RS13865 Protein ID WP_000813794.1
Coordinates 2251064..2251240 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H4F95_RS13870 Protein ID WP_001270286.1
Coordinates 2251286..2251702 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS13845 (2246683) 2246683..2247897 - 1215 WP_001307190.1 BenE family transporter YdcO -
H4F95_RS13850 (2247950) 2247950..2248486 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
H4F95_RS13855 (2248559) 2248559..2250520 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H4F95_RS13860 (2250612) 2250612..2250842 - 231 WP_000494244.1 YncJ family protein -
H4F95_RS13865 (2251064) 2251064..2251240 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H4F95_RS13870 (2251286) 2251286..2251702 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H4F95_RS13875 (2251781) 2251781..2253187 + 1407 WP_000760589.1 PLP-dependent aminotransferase family protein -
H4F95_RS13880 (2253432) 2253432..2254577 + 1146 WP_000047455.1 ABC transporter substrate-binding protein -
H4F95_RS13885 (2254595) 2254595..2255608 + 1014 WP_000220416.1 ABC transporter ATP-binding protein -
H4F95_RS13890 (2255609) 2255609..2256550 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2245378..2246643 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T202748 WP_000813794.1 NZ_CP075061:2251064-2251240 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T202748 NZ_CP099600:5064981-5065259 [Pseudomonas siliginis]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTATGAAACAGGTACGACTCGTGGAATTCGTGCCGACCATGC
AAAACGGCTTTCGCGCATGTTGCAGTTCATGGATCGAGCGACGCTTCCCGCAGACCTGGACTTGCCCGGCTGGCGCCTGC
ATCCGTTGAAAGGTGAGCTGAGCGAATATTGGTCGCTCAGCGTTTCAGGCAACTGGCGAGTGATCTTTCGTTTTGTCGGT
TCGGATATCGAATTGGTCGATTATCTGGACTACCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT202748 WP_001270286.1 NZ_CP075061:2251286-2251702 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT202748 NZ_CP099600:5065274-5065561 [Pseudomonas siliginis]
ATGCCCATGCACAACCCGCCACACCCCGGTGAAACCCTGCTGCTGGATGTCTTGCCCGAGCTTGGTATCAGCGTGAGCGA
ATTGGCCCGGCACCTTGGCTTTGCACGCCCGCATCTTTCGCGCGTACTGCACGGACACGCGCCGATCAGCCCGGATCTGG
CAGTACGACTTGAGCGTGCAGGGATCGGCAAGGCGCGCGTTTGGCTAGGCGTCCAAACCGACTACGATCTTTGGCAAGCG
GAGCACCGTGAGCAACCAATGATAGAACCCCTCGCAGCCCACGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References