Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1255307..1255925 Replicon chromosome
Accession NZ_CP075061
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H4F95_RS09085 Protein ID WP_001291435.1
Coordinates 1255307..1255525 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H4F95_RS09090 Protein ID WP_000344800.1
Coordinates 1255551..1255925 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS09050 (1250596) 1250596..1251168 + 573 WP_000779839.1 YbaY family lipoprotein -
H4F95_RS09055 (1251199) 1251199..1251510 - 312 WP_000409911.1 MGMT family protein -
H4F95_RS09065 (1251889) 1251889..1252242 + 354 WP_000878141.1 DUF1428 family protein -
H4F95_RS09070 (1252284) 1252284..1253834 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H4F95_RS09075 (1253998) 1253998..1254468 - 471 WP_000136192.1 YlaC family protein -
H4F95_RS09080 (1254584) 1254584..1255135 - 552 WP_000102564.1 maltose O-acetyltransferase -
H4F95_RS09085 (1255307) 1255307..1255525 - 219 WP_001291435.1 HHA domain-containing protein Toxin
H4F95_RS09090 (1255551) 1255551..1255925 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H4F95_RS09095 (1256471) 1256471..1259620 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H4F95_RS09100 (1259643) 1259643..1260836 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202747 WP_001291435.1 NZ_CP075061:c1255525-1255307 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T202747 NZ_CP099600:3258371-3258592 [Pseudomonas siliginis]
ATCGAATACGTAGAACAACGCAACACCGTCGCTTCAGTTGCACTGCACCAAAAGCTCAGCGCCGCAGCGCGAAGGCTTTC
CTGGATACCGCATGGTTTCCGCTCCGGCCGAATACCGGGCACTCGCGAAATGGTCATCAACTCGAATTATTTACTGGTCT
ATCAGGTGACAGACCACATCAAAATTCTGACCGTGTTGCACGCCCGTAAAAAATATCCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT202747 WP_000344800.1 NZ_CP075061:c1255925-1255551 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT202747 NZ_CP099600:3258139-3258333 [Pseudomonas siliginis]
ATGAGCGCTGAACTTTCACTTATCGTTTCAGACTTCGAAACCGAAGAAGAGGCTGCGGATTACGATCTTTGGTATCGCGC
CAAGGTACAAGCCGCACTTGATGATCCACGTCCGCCTATCCCCCACGATGAGGCAATGGTGCTGCTAGGTCAGATGCTGG
AAGAGAGACGCAAGAATCGACGCGCTGCCGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References