Detailed information of TA system TA202746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1221009..1221846
• Replicon: chromosome
• Accession: NZ_CP075061
• Organism: Escherichia coli strain TCM_B_2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: H4F95_RS08915
• Protein ID: WP_000227784.1
• Coordinates: 1221009..1221551 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: H4F95_RS08920
• Protein ID: WP_001297137.1
• Coordinates: 1221535..1221846 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F95_RS08895 (1216548)	1216548..1217459	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
H4F95_RS08900 (1217627)	1217627..1218118	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
H4F95_RS08905 (1218246)	1218246..1219610	-	1365	WP_001000978.1	MFS transporter	-
H4F95_RS08910 (1220018)	1220018..1220953	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
H4F95_RS08915 (1221009)	1221009..1221551	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
H4F95_RS08920 (1221535)	1221535..1221846	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
H4F95_RS08925 (1222031)	1222031..1222921	-	891	WP_000971336.1	heme o synthase	-
H4F95_RS08930 (1222933)	1222933..1223262	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
H4F95_RS08935 (1223262)	1223262..1223876	-	615	WP_000179814.1	cytochrome o ubiquinol oxidase subunit III	-
H4F95_RS08940 (1223866)	1223866..1225857	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
H4F95_RS08945 (1225879)	1225879..1226826	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T202746 WP_000227784.1 NZ_CP075061:c1221551-1221009 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T202746 NZ_CP099600:1739315-1739527 [Pseudomonas siliginis]
GTGCAGAGCAGGCTATTGATGAAGGAGCTGGAGGAGGCGGGCTGGACGCTGGATCGGGTTGCGGGCAGTCATCACATCTT
CACTCATCGTTACAACCCGAACACGATTGCCGTCCCGCATCCGAAAAAGGATTTACCGCTGGGGACAGTCAAAAGCATTC
GGCGGCGCGCGGGGTTATTCAGCCCGCCAACCCGTCATGAAGGAGATCCGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT202746 WP_001297137.1 NZ_CP075061:c1221846-1221535 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT202746 NZ_CP099600:1739527-1739931 [Pseudomonas siliginis]
ATGCAATATCCCATCTGCATCGAGTGGGGTGACGAGAACACTGCCATCGGCATTCAGATCCCCGATATTCCCGGCGCAGT
CACGGCCGGGGACAGCTTTGAGGAGGCCTACAACGCGGCCGTGGAAGTGGCTCACCTCATGCTGCAGGAGATCGCGGCGG
CCGGTCAGGCGATTCCGATGCCGACCTCGGCAGCGGCGCATCGCAATCATCCGGATTTTGCCGAGATGGGCTGGGGCATG
CTGGAGCTGGATATCTCGCCGTACCTGGGCAAGACCGAAAAGGTCAACGTTACGCTGCCCGGCTATGTGATTCAGCGTAT
TGATCGCTATGTACGCGAGCACAACGTCAAAAGCCGCTCCTCGTTTCTGGCGGATGCGGCGATGGAGAAACTGGTTCGGT
ATTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3