Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 419458..419964 Replicon chromosome
Accession NZ_CP075061
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag H4F95_RS05055 Protein ID WP_213206492.1
Coordinates 419782..419964 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H4F95_RS05050 Protein ID WP_000398619.1
Coordinates 419458..419754 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS05030 (416189) 416189..416908 - 720 WP_000611288.1 two-component system response regulator DcuR -
H4F95_RS05035 (416905) 416905..418536 - 1632 WP_001216460.1 sensor histidine kinase -
H4F95_RS05040 (418717) 418717..418947 + 231 WP_000371699.1 4Fe-4S mono-cluster protein YjdI -
H4F95_RS05045 (418959) 418959..419231 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
H4F95_RS05050 (419458) 419458..419754 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H4F95_RS05055 (419782) 419782..419964 + 183 WP_213206492.1 type V toxin-antitoxin system toxin GhoT Toxin
H4F95_RS05060 (420074) 420074..421591 - 1518 WP_001295063.1 lysine--tRNA ligase -
H4F95_RS05065 (421828) 421828..423285 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6907.46 Da        Isoelectric Point: 7.1606

>T202743 WP_213206492.1 NZ_CP075061:419782-419964 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHICLLSAFLVGITWPMSLPVALLFSLFYEL

Download         Length: 183 bp

>T202743 NZ_CP099599:5110842-5111120 [Pseudomonas siliginis]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTATGAAACAGGTACGACTCGTGGAATTCGTGCCGACCATGC
AAAACGGCTTTCGCGCATGTTGCAGTTCATGGATCGAGCGACGCTTCCCGCAGACCTGGACTTGCCCGGCTGGCGCCTGC
ATCCGTTGAAAGGTGAGCTGAGCGAATATTGGTCGCTCAGCGTTTCAGGCAACTGGCGAGTGATCTTTCGTTTTGTCGGT
TCGGATATCGAATTGGTCGATTATCTGGACTACCACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT202743 WP_000398619.1 NZ_CP075061:419458-419754 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT202743 NZ_CP099599:5111135-5111422 [Pseudomonas siliginis]
ATGCCCATGCACAACCCGCCACACCCCGGTGAAACCCTGCTGCTGGATGTCTTGCCCGAGCTTGGTATCAGCGTGAGCGA
ATTGGCCCGGCACCTTGGCTTTGCACGCCCGCATCTTTCGCGCGTACTGCACGGACACGCGCCGATCAGCCCGGATCTGG
CAGTACGACTTGAGCGTGCAGGGATCGGCAAGGCGCGCGTTTGGCTAGGCGTCCAAACCGACTACGATCTTTGGCAAGCG
GAGCACCGTGAGCAACCAATGATAGAACCCCTCGCAGCCCACGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References