Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 33599..34397 Replicon plasmid pU_B2_3
Accession NZ_CP075058
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag H4F95_RS01895 Protein ID WP_000072677.1
Coordinates 33876..34397 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag H4F95_RS01890 Protein ID WP_001351987.1
Coordinates 33599..33868 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS01865 (H4F95_01865) 28720..30060 - 1341 WP_000137333.1 DnaB-like helicase C-terminal domain-containing protein -
H4F95_RS01870 (H4F95_01870) 30104..30844 - 741 WP_063277813.1 hypothetical protein -
H4F95_RS01875 (H4F95_01875) 31134..32192 + 1059 WP_021512318.1 hypothetical protein -
H4F95_RS01880 (H4F95_01880) 32253..32606 - 354 WP_160394000.1 hypothetical protein -
H4F95_RS01885 (H4F95_01885) 32612..33280 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
H4F95_RS01890 (H4F95_01890) 33599..33868 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
H4F95_RS01895 (H4F95_01895) 33876..34397 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
H4F95_RS01900 (H4F95_01900) 34566..34817 - 252 WP_000901559.1 hypothetical protein -
H4F95_RS01905 (H4F95_01905) 34819..35511 - 693 WP_000856757.1 hypothetical protein -
H4F95_RS01910 (H4F95_01910) 35525..35848 - 324 WP_000064175.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..107542 107542


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T202736 WP_000072677.1 NZ_CP075058:33876-34397 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T202736 NZ_CP099598:3285040-3285261 [Pseudomonas siliginis]
ATCGAATACGTAGAACAACGCAACACTGTCGCTTCAGTTGCACTGCACCAAAAGCTCAGCGCCGCAGCGCGAAGGCTTTC
CTGGATACCGCATGGTTTCCGCTCCGGCCGAATACCGGGCACTCGCGAAATGGTCATCAACTCGAATTATTTACTGGTCT
ATCAGGTGACAGACCACATCAAAATTCTGACCGTGTTGCACGCCCGTAAAAAATATCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT202736 WP_001351987.1 NZ_CP075058:33599-33868 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT202736 NZ_CP099598:3284808-3285002 [Pseudomonas siliginis]
ATGAGCGCTGAACTTTCACTTATCGTTTCAGACTTCGAAACCGAAGAAGAGGCTGCGGATTACGATCTTTGGTATCGCGC
CAAGGTACAAGCCGCACTTGATGATCCACGTCCGCCTATCCCCCACGATGAGGCAATGGTGCTGCTAGGTCAGATGCTGG
AAGAGAGACGCAAGAATCGACGCGCTGCCGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References