Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | ataT-KacA/DUF1778(antitoxin) |
Location | 33599..34397 | Replicon | plasmid pU_B2_3 |
Accession | NZ_CP075058 | ||
Organism | Escherichia coli strain TCM_B_2 |
Toxin (Protein)
Gene name | ataT | Uniprot ID | E2QDF3 |
Locus tag | H4F95_RS01895 | Protein ID | WP_000072677.1 |
Coordinates | 33876..34397 (+) | Length | 174 a.a. |
Antitoxin (Protein)
Gene name | KacA | Uniprot ID | A0A0V9R6Q7 |
Locus tag | H4F95_RS01890 | Protein ID | WP_001351987.1 |
Coordinates | 33599..33868 (+) | Length | 90 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
H4F95_RS01865 (H4F95_01865) | 28720..30060 | - | 1341 | WP_000137333.1 | DnaB-like helicase C-terminal domain-containing protein | - |
H4F95_RS01870 (H4F95_01870) | 30104..30844 | - | 741 | WP_063277813.1 | hypothetical protein | - |
H4F95_RS01875 (H4F95_01875) | 31134..32192 | + | 1059 | WP_021512318.1 | hypothetical protein | - |
H4F95_RS01880 (H4F95_01880) | 32253..32606 | - | 354 | WP_160394000.1 | hypothetical protein | - |
H4F95_RS01885 (H4F95_01885) | 32612..33280 | - | 669 | WP_000161228.1 | division plane positioning ATPase MipZ | - |
H4F95_RS01890 (H4F95_01890) | 33599..33868 | + | 270 | WP_001351987.1 | DUF1778 domain-containing protein | Antitoxin |
H4F95_RS01895 (H4F95_01895) | 33876..34397 | + | 522 | WP_000072677.1 | GNAT family N-acetyltransferase | Toxin |
H4F95_RS01900 (H4F95_01900) | 34566..34817 | - | 252 | WP_000901559.1 | hypothetical protein | - |
H4F95_RS01905 (H4F95_01905) | 34819..35511 | - | 693 | WP_000856757.1 | hypothetical protein | - |
H4F95_RS01910 (H4F95_01910) | 35525..35848 | - | 324 | WP_000064175.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | - | 1..107542 | 107542 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 174 a.a. Molecular weight: 19392.17 Da Isoelectric Point: 8.6369
>T202736 WP_000072677.1 NZ_CP075058:33876-34397 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE
Download Length: 522 bp
>T202736 NZ_CP099598:3285040-3285261 [Pseudomonas siliginis]
ATCGAATACGTAGAACAACGCAACACTGTCGCTTCAGTTGCACTGCACCAAAAGCTCAGCGCCGCAGCGCGAAGGCTTTC
CTGGATACCGCATGGTTTCCGCTCCGGCCGAATACCGGGCACTCGCGAAATGGTCATCAACTCGAATTATTTACTGGTCT
ATCAGGTGACAGACCACATCAAAATTCTGACCGTGTTGCACGCCCGTAAAAAATATCCATAA
ATCGAATACGTAGAACAACGCAACACTGTCGCTTCAGTTGCACTGCACCAAAAGCTCAGCGCCGCAGCGCGAAGGCTTTC
CTGGATACCGCATGGTTTCCGCTCCGGCCGAATACCGGGCACTCGCGAAATGGTCATCAACTCGAATTATTTACTGGTCT
ATCAGGTGACAGACCACATCAAAATTCTGACCGTGTTGCACGCCCGTAAAAAATATCCATAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10082.54 Da Isoelectric Point: 7.0155
>AT202736 WP_001351987.1 NZ_CP075058:33599-33868 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME
Download Length: 270 bp
>AT202736 NZ_CP099598:3284808-3285002 [Pseudomonas siliginis]
ATGAGCGCTGAACTTTCACTTATCGTTTCAGACTTCGAAACCGAAGAAGAGGCTGCGGATTACGATCTTTGGTATCGCGC
CAAGGTACAAGCCGCACTTGATGATCCACGTCCGCCTATCCCCCACGATGAGGCAATGGTGCTGCTAGGTCAGATGCTGG
AAGAGAGACGCAAGAATCGACGCGCTGCCGGTTAG
ATGAGCGCTGAACTTTCACTTATCGTTTCAGACTTCGAAACCGAAGAAGAGGCTGCGGATTACGATCTTTGGTATCGCGC
CAAGGTACAAGCCGCACTTGATGATCCACGTCCGCCTATCCCCCACGATGAGGCAATGGTGCTGCTAGGTCAGATGCTGG
AAGAGAGACGCAAGAATCGACGCGCTGCCGGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0V9R6P3 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0V9R6Q7 |