Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 93077..93720 Replicon plasmid pB2_2
Accession NZ_CP075057
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag H4F95_RS01630 Protein ID WP_001044768.1
Coordinates 93304..93720 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag H4F95_RS01625 Protein ID WP_001261287.1
Coordinates 93077..93307 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS01615 (88255) 88255..89388 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
H4F95_RS01620 (89651) 89651..92770 - 3120 WP_023909028.1 hypothetical protein -
H4F95_RS01625 (93077) 93077..93307 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H4F95_RS01630 (93304) 93304..93720 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
H4F95_RS01635 (93882) 93882..96020 - 2139 WP_202757506.1 AAA family ATPase -
H4F95_RS01640 (96485) 96485..97498 + 1014 WP_085674723.1 nucleotidyltransferase -
H4F95_RS01645 (97541) 97541..98434 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / aac(6')-Ib-cr / dfrA17 / aadA5 / qacE / mph(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId - 1..108031 108031
- flank IS/Tn - - 98571..99074 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T202735 WP_001044768.1 NZ_CP075057:93304-93720 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T202735 NZ_CP099598:c2254959-2254690 [Pseudomonas siliginis]
ATGCAGATTGAGTGGCGTCCCGAAGCCAAGGCCGATCTTTCGAACATCCTCAGATACATTGGCGAGAGAAACTTTGCAGC
CGCAGTGAGTCTCTCGAAATCCATCGAAGAATCCACAACCTCACTCTCCGTTCATCCCCATCTCTACCGTAAAGGTCGAT
CTCCCGGCACCCGGGAAATCGTTGTACATCCTAACTACGTAGTGATTTATGAGGTGACGGATCGAATCGAGATACTTAGC
GTTTTGCATACCCGACAGGAATATCCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT202735 WP_001261287.1 NZ_CP075057:93077-93307 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT202735 NZ_CP099598:c2255140-2254949 [Pseudomonas siliginis]
ATGGACGGCAAAATCTTTCCCGACGATTCAGGGTTTAAAAACAACGAGCAGGCTGCCAGTCATGATCGCTGGTTGCGAGC
CAAGGTGCAGGCGTCCAGAGATGATCCCCATCCGAGTCTCCCCCATGAGGACGTGATGGCGGACATGCATGCCTTGATCG
AATCCATGCGAAAAAAGGTCGATGCAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References