Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | vagCD/VapC-VagC |
Location | 93077..93720 | Replicon | plasmid pB2_2 |
Accession | NZ_CP075057 | ||
Organism | Escherichia coli strain TCM_B_2 |
Toxin (Protein)
Gene name | vagD | Uniprot ID | B1LRW4 |
Locus tag | H4F95_RS01630 | Protein ID | WP_001044768.1 |
Coordinates | 93304..93720 (+) | Length | 139 a.a. |
Antitoxin (Protein)
Gene name | vagC | Uniprot ID | D2WFK3 |
Locus tag | H4F95_RS01625 | Protein ID | WP_001261287.1 |
Coordinates | 93077..93307 (+) | Length | 77 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
H4F95_RS01615 (88255) | 88255..89388 | - | 1134 | WP_000545983.1 | DUF3800 domain-containing protein | - |
H4F95_RS01620 (89651) | 89651..92770 | - | 3120 | WP_023909028.1 | hypothetical protein | - |
H4F95_RS01625 (93077) | 93077..93307 | + | 231 | WP_001261287.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
H4F95_RS01630 (93304) | 93304..93720 | + | 417 | WP_001044768.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
H4F95_RS01635 (93882) | 93882..96020 | - | 2139 | WP_202757506.1 | AAA family ATPase | - |
H4F95_RS01640 (96485) | 96485..97498 | + | 1014 | WP_085674723.1 | nucleotidyltransferase | - |
H4F95_RS01645 (97541) | 97541..98434 | + | 894 | WP_001553853.1 | S-4TM family putative pore-forming effector | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | tet(B) / aac(6')-Ib-cr / dfrA17 / aadA5 / qacE / mph(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId | - | 1..108031 | 108031 | |
- | flank | IS/Tn | - | - | 98571..99074 | 503 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15197.66 Da Isoelectric Point: 7.7805
>T202735 WP_001044768.1 NZ_CP075057:93304-93720 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK
Download Length: 417 bp
>T202735 NZ_CP099598:c2254959-2254690 [Pseudomonas siliginis]
ATGCAGATTGAGTGGCGTCCCGAAGCCAAGGCCGATCTTTCGAACATCCTCAGATACATTGGCGAGAGAAACTTTGCAGC
CGCAGTGAGTCTCTCGAAATCCATCGAAGAATCCACAACCTCACTCTCCGTTCATCCCCATCTCTACCGTAAAGGTCGAT
CTCCCGGCACCCGGGAAATCGTTGTACATCCTAACTACGTAGTGATTTATGAGGTGACGGATCGAATCGAGATACTTAGC
GTTTTGCATACCCGACAGGAATATCCATAA
ATGCAGATTGAGTGGCGTCCCGAAGCCAAGGCCGATCTTTCGAACATCCTCAGATACATTGGCGAGAGAAACTTTGCAGC
CGCAGTGAGTCTCTCGAAATCCATCGAAGAATCCACAACCTCACTCTCCGTTCATCCCCATCTCTACCGTAAAGGTCGAT
CTCCCGGCACCCGGGAAATCGTTGTACATCCTAACTACGTAGTGATTTATGAGGTGACGGATCGAATCGAGATACTTAGC
GTTTTGCATACCCGACAGGAATATCCATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8815.90 Da Isoelectric Point: 4.3775
>AT202735 WP_001261287.1 NZ_CP075057:93077-93307 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT202735 NZ_CP099598:c2255140-2254949 [Pseudomonas siliginis]
ATGGACGGCAAAATCTTTCCCGACGATTCAGGGTTTAAAAACAACGAGCAGGCTGCCAGTCATGATCGCTGGTTGCGAGC
CAAGGTGCAGGCGTCCAGAGATGATCCCCATCCGAGTCTCCCCCATGAGGACGTGATGGCGGACATGCATGCCTTGATCG
AATCCATGCGAAAAAAGGTCGATGCAGATTGA
ATGGACGGCAAAATCTTTCCCGACGATTCAGGGTTTAAAAACAACGAGCAGGCTGCCAGTCATGATCGCTGGTTGCGAGC
CAAGGTGCAGGCGTCCAGAGATGATCCCCATCCGAGTCTCCCCCATGAGGACGTGATGGCGGACATGCATGCCTTGATCG
AATCCATGCGAAAAAAGGTCGATGCAGATTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A606Q844 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7U9QFC4 |