Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | hipBA/HipA-HipB |
Location | 87763..89386 | Replicon | plasmid pMCR_B2_1 |
Accession | NZ_CP075056 | ||
Organism | Escherichia coli strain TCM_B_2 |
Toxin (Protein)
Gene name | hipA | Uniprot ID | H9TH67 |
Locus tag | H4F95_RS00420 | Protein ID | WP_000088046.1 |
Coordinates | 88061..89386 (+) | Length | 442 a.a. |
Antitoxin (Protein)
Gene name | hipB | Uniprot ID | A0A5Z6V2D1 |
Locus tag | H4F95_RS00415 | Protein ID | WP_024136327.1 |
Coordinates | 87763..88056 (+) | Length | 98 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
H4F95_RS00390 (H4F95_00390) | 83635..84876 | + | 1242 | WP_001053338.1 | VWA domain-containing protein | - |
H4F95_RS26545 | 84967..85098 | + | 132 | WP_000374059.1 | hypothetical protein | - |
H4F95_RS00395 (H4F95_00395) | 85439..85720 | - | 282 | WP_000077926.1 | hypothetical protein | - |
H4F95_RS00400 (H4F95_00400) | 85770..85961 | - | 192 | WP_000398480.1 | hypothetical protein | - |
H4F95_RS00405 (H4F95_00405) | 86053..86394 | - | 342 | WP_001151575.1 | hypothetical protein | - |
H4F95_RS00410 (H4F95_00410) | 86767..87159 | + | 393 | WP_000341066.1 | hypothetical protein | - |
H4F95_RS00415 (H4F95_00415) | 87763..88056 | + | 294 | WP_024136327.1 | helix-turn-helix transcriptional regulator | Antitoxin |
H4F95_RS00420 (H4F95_00420) | 88061..89386 | + | 1326 | WP_000088046.1 | type II toxin-antitoxin system HipA family toxin | Toxin |
H4F95_RS00425 (H4F95_00425) | 89447..89653 | + | 207 | WP_000134171.1 | hypothetical protein | - |
H4F95_RS00430 (H4F95_00430) | 89755..90165 | + | 411 | WP_000985911.1 | hypothetical protein | - |
H4F95_RS00435 (H4F95_00435) | 90178..90993 | + | 816 | WP_053276223.1 | HNH endonuclease signature motif containing protein | - |
H4F95_RS00440 (H4F95_00440) | 91247..91672 | + | 426 | WP_001043843.1 | hypothetical protein | - |
H4F95_RS00445 (H4F95_00445) | 92221..92529 | + | 309 | WP_001224687.1 | hypothetical protein | - |
H4F95_RS00450 (H4F95_00450) | 92545..93402 | + | 858 | WP_000784387.1 | HNH endonuclease signature motif containing protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | mcr-1.1 / fosA3 / blaCTX-M-14 / aph(3')-Ia | - | 1..209088 | 209088 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 442 a.a. Molecular weight: 50249.20 Da Isoelectric Point: 6.1927
>T202731 WP_000088046.1 NZ_CP075056:88061-89386 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
Download Length: 1326 bp
>T202731 NZ_CP099598:174520-174801 [Pseudomonas siliginis]
ATGACTTATAAGCTTGAGTTTCTGCCGTCTGCGCACAAAGAGTGGAACAAGCTGGGTCATACGTTGCGGGAGCAGTTCAA
AAAGAAGCTGGGTGAGAGGCTGAAGCTTCCCAGAATCCCGGCTGACGCCCTCCACAGCATGCCCGACTGCTACAAAATCA
AACTCAAAGCCTCGGGATATCGATTGGTTTACCAAGTCATCGACGAACGAGTCGTGGTTTCCGTGCTAGCGGTTGGCAAG
CGCGAACGCAGCAGCGTTTACGATGCTGCCAGGAACCGTTGA
ATGACTTATAAGCTTGAGTTTCTGCCGTCTGCGCACAAAGAGTGGAACAAGCTGGGTCATACGTTGCGGGAGCAGTTCAA
AAAGAAGCTGGGTGAGAGGCTGAAGCTTCCCAGAATCCCGGCTGACGCCCTCCACAGCATGCCCGACTGCTACAAAATCA
AACTCAAAGCCTCGGGATATCGATTGGTTTACCAAGTCATCGACGAACGAGTCGTGGTTTCCGTGCTAGCGGTTGGCAAG
CGCGAACGCAGCAGCGTTTACGATGCTGCCAGGAACCGTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 10946.35 Da Isoelectric Point: 10.0925
>AT202731 WP_024136327.1 NZ_CP075056:87763-88056 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW
Download Length: 294 bp
>AT202731 NZ_CP099598:174285-174530 [Pseudomonas siliginis]
ATGCAAAGCGTTCTCGCCGATATGGCTGTCAGTGTCTCTGAACTGAAGAAAAATCCTACTGCCGTGTTGAACGGTGCACA
TGGGGGGGCCGTCGCTGTCCTCAACCACAATCGCGTCATGGGCTATATGGTGCCGGCCGAAGTTTATGAAGCAATGATGG
AGCGGCTTGATGATCTTGAGCTGGCTGACATTGTTCGTTCCCGCCTCCACGAAACGCCCATTTCGGTAAGCCTGGATGAC
TTATAA
ATGCAAAGCGTTCTCGCCGATATGGCTGTCAGTGTCTCTGAACTGAAGAAAAATCCTACTGCCGTGTTGAACGGTGCACA
TGGGGGGGCCGTCGCTGTCCTCAACCACAATCGCGTCATGGGCTATATGGTGCCGGCCGAAGTTTATGAAGCAATGATGG
AGCGGCTTGATGATCTTGAGCTGGCTGACATTGTTCGTTCCCGCCTCCACGAAACGCCCATTTCGGTAAGCCTGGATGAC
TTATAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A3T0VBU1 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A5Z6V2D1 |