Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 3466907..3467544 | Replicon | chromosome |
| Accession | NZ_CP075054 | ||
| Organism | Bacillus velezensis strain VTX20 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | KH277_RS17035 | Protein ID | WP_003156187.1 |
| Coordinates | 3466907..3467257 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | KH277_RS17040 | Protein ID | WP_003156188.1 |
| Coordinates | 3467263..3467544 (-) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KH277_RS16995 (KH277_16985) | 3461947..3462549 | - | 603 | WP_003156170.1 | PP2C family serine/threonine-protein phosphatase | - |
| KH277_RS17000 (KH277_16990) | 3462549..3463337 | - | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| KH277_RS17005 (KH277_16995) | 3463303..3463785 | - | 483 | WP_003156173.1 | anti-sigma B factor RsbW | - |
| KH277_RS17010 (KH277_17000) | 3463782..3464111 | - | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| KH277_RS17015 (KH277_17005) | 3464175..3465182 | - | 1008 | WP_025853725.1 | PP2C family protein-serine/threonine phosphatase | - |
| KH277_RS17020 (KH277_17010) | 3465194..3465595 | - | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| KH277_RS17025 (KH277_17015) | 3465598..3465963 | - | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| KH277_RS17030 (KH277_17020) | 3465968..3466789 | - | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| KH277_RS17035 (KH277_17025) | 3466907..3467257 | - | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KH277_RS17040 (KH277_17030) | 3467263..3467544 | - | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| KH277_RS17045 (KH277_17035) | 3467664..3468833 | - | 1170 | WP_003156189.1 | alanine racemase | - |
| KH277_RS17050 (KH277_17040) | 3468950..3469957 | - | 1008 | WP_003156191.1 | outer membrane lipoprotein carrier protein LolA | - |
| KH277_RS17055 (KH277_17045) | 3470122..3470487 | - | 366 | WP_003156192.1 | holo-ACP synthase | - |
| KH277_RS17060 (KH277_17050) | 3470580..3471179 | + | 600 | WP_015388676.1 | rhomboid family intramembrane serine protease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T202728 WP_003156187.1 NZ_CP075054:c3467257-3466907 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T202728 NZ_CP099596:c2317368-2317084 [Pseudomonas siliginis]
ATGCAGATTAAGTGGCGACCCAAGGCCCGGGTCGAACTCTCGAAAATCCTGAAGTACATTGGCGAGCGAAATTTTGAAGC
GGCGACTGCACTTCACAAGTCCGTCGTGAAAGCTACATCTGCGCTTTGTTGGCATCCACACCTGTATCGCAAAGGCCGCT
CTCCCGGCACTCGAGAGATTGTGGTAAGCCCCAACTATCTCGTCGTTTATCAAGTGGCGGACCACATTGAGATTGTGAGC
ATCTTGCATGCGCGTCAGGAGTACCCTCGACGCGACCCCGGTTAA
ATGCAGATTAAGTGGCGACCCAAGGCCCGGGTCGAACTCTCGAAAATCCTGAAGTACATTGGCGAGCGAAATTTTGAAGC
GGCGACTGCACTTCACAAGTCCGTCGTGAAAGCTACATCTGCGCTTTGTTGGCATCCACACCTGTATCGCAAAGGCCGCT
CTCCCGGCACTCGAGAGATTGTGGTAAGCCCCAACTATCTCGTCGTTTATCAAGTGGCGGACCACATTGAGATTGTGAGC
ATCTTGCATGCGCGTCAGGAGTACCCTCGACGCGACCCCGGTTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT202728 WP_003156188.1 NZ_CP075054:c3467544-3467263 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT202728 NZ_CP099596:c2317564-2317358 [Pseudomonas siliginis]
ATGATCGACCCGTCCTTGATCGACCCACTATGCCCGGTTGTTTCGGATCTTGATCCCGAGGAGCAGGCTGCGAGTTATGA
CCGCTGGTTTCGGGCCGAAGTACAGGCCTCTCTCGATGATCCGCGTCCGAGTATTCCTCATGAGCAAGTGATGGAGGAAA
TGAATGCCTTGATGGAATCTATGCGCAAAAGCGCAGATGCAGATTAA
ATGATCGACCCGTCCTTGATCGACCCACTATGCCCGGTTGTTTCGGATCTTGATCCCGAGGAGCAGGCTGCGAGTTATGA
CCGCTGGTTTCGGGCCGAAGTACAGGCCTCTCTCGATGATCCGCGTCCGAGTATTCCTCATGAGCAAGTGATGGAGGAAA
TGAATGCCTTGATGGAATCTATGCGCAAAAGCGCAGATGCAGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|