Detailed information of TA system TA202727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2664997..2665914
• Replicon: chromosome
• Accession: NZ_CP075054
• Organism: Bacillus velezensis strain VTX20

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KH277_RS12725
• Protein ID: WP_025851786.1
• Coordinates: 2664997..2665743 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KH277_RS12730
• Protein ID: WP_003154807.1
• Coordinates: 2665744..2665914 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH277_RS12705 (KH277_12705)	2660390..2661340	-	951	WP_015388352.1	ring-cleaving dioxygenase	-
KH277_RS12710 (KH277_12710)	2661663..2662979	+	1317	WP_003154801.1	amino acid permease	-
KH277_RS12715 (KH277_12715)	2663265..2663882	+	618	WP_003154804.1	DUF47 domain-containing protein	-
KH277_RS12720 (KH277_12720)	2663895..2664893	+	999	WP_003154805.1	inorganic phosphate transporter	-
KH277_RS12725 (KH277_12725)	2664997..2665743	+	747	WP_025851786.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KH277_RS12730 (KH277_12730)	2665744..2665914	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KH277_RS19250	2666011..2666136	+	126	WP_003154809.1	hypothetical protein	-
KH277_RS12735 (KH277_12735)	2666172..2667050	-	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
KH277_RS12740 (KH277_12740)	2667064..2667327	-	264	WP_003154813.1	phage holin	-
KH277_RS12745 (KH277_12745)	2667341..2667604	-	264	WP_003154815.1	hemolysin XhlA family protein	-
KH277_RS12750 (KH277_12750)	2667656..2668417	-	762	WP_043867020.1	hypothetical protein	-
KH277_RS12755 (KH277_12755)	2668474..2668671	-	198	WP_003154819.1	XkdX family protein	-
KH277_RS12760 (KH277_12760)	2668677..2669048	-	372	WP_014304856.1	XkdW family protein	-
KH277_RS12765 (KH277_12765)	2669061..2670683	-	1623	WP_003154821.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29048.53 Da
• Isoelectric Point: 4.7003

>T202727 WP_025851786.1 NZ_CP075054:2664997-2665743 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T202727 NZ_CP099596:1766739-1766951 [Pseudomonas siliginis]
GTGCAGAGCAGGCTATTGATGAAGGAGCTGGAGGAGGCGGGCTGGACGCTGGATCGGGTTGCGGGCAGTCATCACATCTT
CACTCATCGTTACAACCCGAACACGATTGCCGTCCCGCATCCGAAAAAGGATTTACCGTTGGGGACAGTCAAAAGCATTC
GGCGGCGCGCGGGGTTATTCAGCCCGCCAACCCGTCATGAAGGAGATCCGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT202727 WP_003154807.1 NZ_CP075054:2665744-2665914 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT202727 NZ_CP099596:1766951-1767355 [Pseudomonas siliginis]
ATGCAATATCCCATCTGCATCGAGTGGGGTGACGAGAACACTGCCATCGGCATTCAGATCCCCGATATTCCCGGCGCAGT
CACGGCCGGGGACAGCTTTGAGGAGGCCTACAACGCGGCCGTGGAAGTGGCTCACCTCATGCTGCAGGAGATCGCGGCGG
CCGGTCAGGCGATTCCGATGCCGACCTCGGCAGCGGCGCATCGCAATCATCCGGATTTTGCCGAGATGGGCTGGGGCATG
CTGGAGCTGGATATCTCGCCGTACCTGGGCAAGACCGAAAAGGTCAACGTTACGCTGCCCGGCTATGTGATTCAGCGTAT
TGATCGCTATGTACGCGAGCATAACGTCAAAAGCCGCTCCTCGTTTCTGGCGGATGCGGCGATGGAGAAACTGGTTCGGT
ATTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14