Detailed information of TA system TA202725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 108699..109224
• Replicon: plasmid pCFSAN012622
• Accession: NZ_CP075038
• Organism: Salmonella enterica subsp. enterica serovar Give strain CFSAN012622

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A8E7KDP4
• Locus tag: GJ28_RS22740
• Protein ID: WP_031570338.1
• Coordinates: 108919..109224 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A8E7KCT6
• Locus tag: GJ28_RS22735
• Protein ID: WP_031570335.1
• Coordinates: 108699..108917 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ28_RS22715 (104639)	104639..105457	-	819	WP_031570318.1	polysaccharide deacetylase family protein	-
GJ28_RS22720 (105487)	105487..105723	-	237	WP_031570321.1	hypothetical protein	-
GJ28_RS22725 (105844)	105844..106182	-	339	WP_031615773.1	helix-turn-helix transcriptional regulator	-
GJ28_RS22730 (107556)	107556..108026	-	471	WP_031570332.1	DNA-binding protein	-
GJ28_RS22735 (108699)	108699..108917	+	219	WP_031570335.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
GJ28_RS22740 (108919)	108919..109224	+	306	WP_031570338.1	type II toxin-antitoxin system toxin CcdB	Toxin
GJ28_RS22745 (109226)	109226..109516	+	291	WP_001266178.1	hypothetical protein	-
GJ28_RS22750 (109513)	109513..109977	+	465	Protein_123	cytoplasmic protein	-
GJ28_RS22755 (110012)	110012..110794	+	783	WP_031570341.1	site-specific integrase	-
GJ28_RS22760 (110945)	110945..111886	-	942	WP_000728920.1	Rpn family recombination-promoting nuclease/putative transposase	-
GJ28_RS22765 (112419)	112419..112685	+	267	WP_149027841.1	hypothetical protein	-
GJ28_RS22770 (112693)	112693..113100	+	408	WP_031615774.1	hypothetical protein	-
GJ28_RS22775 (113116)	113116..113706	+	591	WP_031570346.1	hypothetical protein	-
GJ28_RS22780 (113799)	113799..114032	-	234	WP_254891924.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeC / faeD / faeE / faeH / faeI / faeJ / invA / invC/sctN / spaP / spaQ / prgI / papD / papC	1..147099	147099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11516.31 Da
• Isoelectric Point: 5.1152

>T202725 WP_031570338.1 NZ_CP075038:108919-109224 [Salmonella enterica subsp. enterica serovar Give]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSDKVPRDLYPVMHVGDEPYRLLTTDMASVPASVIGEEV
ADLSLQENDIRDAINLMFRGI

Nucleotide

• Length: 306 bp

>T202725 NZ_CP099596:178024-178305 [Pseudomonas siliginis]
ATGACTTATAAGCTTGAGTTTCTGCCGTCTGCGCACAAAGAGTGGAACAAGCTGGGTCATACGTTGCGGGAGCAGTTCAA
AAAGAAGCTGGGTGAGAGGCTGAAGCTTCCCAGAATCCCGGCTGACGCCCTCCATAGCATGCCCGACTGCTACAAAATCA
AACTCAAAGCCTCGGGATATCGATTGGTTTACCAAGTCATCGACGAACGAGTCGTGGTTTCCGTGCTAGCGGTTGGCAAG
CGCGAACGCAGCAGCGTTTACGATGCTGCCAGGAACCGTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8329.17 Da
• Isoelectric Point: 4.4013

>AT202725 WP_031570335.1 NZ_CP075038:108699-108917 [Salmonella enterica subsp. enterica serovar Give]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEADGSFADENRNW

Nucleotide

• Length: 219 bp

>AT202725 NZ_CP099596:177789-178034 [Pseudomonas siliginis]
ATGCAAAGCGTTCTCGCCGATATGGCTGTCAGTGTCTCTGAACTGAAGAAAAATCCTACTGCCGTGTTGAACGGTGCACA
TGGGGGGGCCGTCGCTGTCCTCAACCACAATCGCGTCATGGGCTATATGGTGCCGGCCGAAGTTTATGAAGCAATGATGG
AGCGGCTTGATGATCTTGAGCTGGCTGACATTGTTCGTTCCCGCCTCCACGAAACGCCCATTTCGGTAAGCCTGGATGAC
TTATAA