Detailed information of TA system TA202721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4132494..4133275
• Replicon: chromosome
• Accession: NZ_CP075037
• Organism: Salmonella enterica subsp. enterica serovar Give strain CFSAN012622

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A0R9NZI3
• Locus tag: GJ28_RS19970
• Protein ID: WP_000625912.1
• Coordinates: 4132494..4132985 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: M7RNV8
• Locus tag: GJ28_RS19975
• Protein ID: WP_001110450.1
• Coordinates: 4132982..4133275 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ28_RS19945 (4127630)	4127630..4130068	-	2439	WP_031569791.1	F4 (K88) fimbrial usher FaeD	-
GJ28_RS19950 (4130078)	4130078..4130617	-	540	WP_000721295.1	type 1 fimbrial protein	-
GJ28_RS19955 (4130652)	4130652..4130936	-	285	WP_192917775.1	PapB/FocB family fimbrial expression transcriptional regulator	-
GJ28_RS19960 (4131529)	4131529..4131756	+	228	WP_001112992.1	hypothetical protein	-
GJ28_RS19965 (4132031)	4132031..4132279	-	249	Protein_3924	IS481 family transposase	-
GJ28_RS19970 (4132494)	4132494..4132985	-	492	WP_000625912.1	GNAT family N-acetyltransferase	Toxin
GJ28_RS19975 (4132982)	4132982..4133275	-	294	WP_001110450.1	DUF1778 domain-containing protein	Antitoxin
GJ28_RS23230 (4133592)	4133592..4133814	+	223	Protein_3927	hypothetical protein	-
GJ28_RS19980 (4134078)	4134078..4134953	+	876	WP_000921676.1	AraC family transcriptional regulator	-
GJ28_RS19985 (4134950)	4134950..4135237	+	288	WP_001269916.1	transcriptional regulator RtsB	-
GJ28_RS23235 (4135230)	4135230..4135412	-	183	WP_001527266.1	ATP-binding cassette domain-containing protein	-
GJ28_RS23240 (4135432)	4135432..4135531	+	100	Protein_3931	hypothetical protein	-
GJ28_RS23245 (4135641)	4135641..4135775	+	135	Protein_3932	hypothetical protein	-
GJ28_RS19995 (4136070)	4136070..4136975	-	906	WP_001268209.1	YjiK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	faeI / faeH / faeF / faeE / faeD / faeC	4120001..4135412	15411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17606.43 Da
• Isoelectric Point: 7.7297

>T202721 WP_000625912.1 NZ_CP075037:c4132985-4132494 [Salmonella enterica subsp. enterica serovar Give]
MISSPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQTTGASRTFVCCGSDSSVLAYYSLASSAVTTNTAPGRFRRNMPDP
IPIVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYQRVGFVPSPMDPMMLMVTLGDLV
ESV

Nucleotide

• Length: 492 bp

>T202721 NZ_CP099590:c4199535-4199362 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10949.56 Da
• Isoelectric Point: 8.6141

>AT202721 WP_001110450.1 NZ_CP075037:c4133275-4132982 [Salmonella enterica subsp. enterica serovar Give]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLARLDQTPSPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT202721 NZ_CP099590:c4199859-4199563 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0R9NZI3

Antitoxin

• AlphaFold DB: A0A0R9PGG6