Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 270081..270723 Replicon chromosome
Accession NZ_CP074714
Organism Bacillus thuringiensis strain B13

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag JRY14_RS02810 Protein ID WP_000635965.1
Coordinates 270373..270723 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag JRY14_RS02805 Protein ID WP_000004570.1
Coordinates 270081..270368 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRY14_RS02780 (JRY14_02750) 265398..266360 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
JRY14_RS02785 (JRY14_02755) 266353..266925 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
JRY14_RS02790 (JRY14_02760) 267019..267378 + 360 WP_000583415.1 holo-ACP synthase -
JRY14_RS02795 (JRY14_02765) 267535..268485 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
JRY14_RS02800 (JRY14_02770) 268603..269772 + 1170 WP_000390615.1 alanine racemase -
JRY14_RS02805 (JRY14_02775) 270081..270368 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
JRY14_RS02810 (JRY14_02780) 270373..270723 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JRY14_RS02815 (JRY14_02785) 270791..272959 + 2169 WP_140159320.1 Tex family protein -
JRY14_RS02820 (JRY14_02790) 273017..273133 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
JRY14_RS02825 (JRY14_02795) 273323..273615 + 293 Protein_256 SprT family protein -
JRY14_RS02830 (JRY14_02800) 273671..275106 + 1436 Protein_257 IS4 family transposase -
JRY14_RS02835 (JRY14_02805) 275236..275457 + 222 Protein_258 zinc ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 273671..274885 1214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T202704 WP_000635965.1 NZ_CP074714:270373-270723 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T202704 NZ_CP099590:c1113753-1113424 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT202704 WP_000004570.1 NZ_CP074714:270081-270368 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT202704 NZ_CP099590:c1114091-1113774 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References