Detailed information of TA system TA202703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 6104..6692
• Replicon: plasmid unnamed4
• Accession: NZ_CP074712
• Organism: Acinetobacter baumannii strain ATCC 17978

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: KIP77_RS20165
• Protein ID: WP_000438826.1
• Coordinates: 6405..6692 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A858UV00
• Locus tag: KIP77_RS20160
• Protein ID: WP_061855757.1
• Coordinates: 6104..6418 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIP77_RS20140 (KIP77_20005)	2079..2450	+	372	WP_000504218.1	hypothetical protein	-
KIP77_RS20145 (KIP77_20010)	2450..2647	+	198	WP_001282483.1	hypothetical protein	-
KIP77_RS20150 (KIP77_20015)	2769..3467	-	699	WP_000007662.1	CPBP family glutamic-type intramembrane protease	-
KIP77_RS20155 (KIP77_20020)	3564..5975	-	2412	WP_000932945.1	TonB-dependent receptor ZnuD2	-
KIP77_RS20160 (KIP77_20025)	6104..6418	-	315	WP_061855757.1	BrnA antitoxin family protein	Antitoxin
KIP77_RS20165 (KIP77_20030)	6405..6692	-	288	WP_000438826.1	BrnT family toxin	Toxin
KIP77_RS20170 (KIP77_20035)	7039..7812	-	774	WP_061855758.1	glucose 1-dehydrogenase	-
KIP77_RS20175 (KIP77_20040)	7910..9112	-	1203	WP_061855756.1	alpha/beta fold hydrolase	-
KIP77_RS20180 (KIP77_20045)	9330..9623	-	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KIP77_RS20185 (KIP77_20050)	9613..9858	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KIP77_RS20190 (KIP77_20055)	10102..10305	+	204	WP_000482656.1	hypothetical protein	-
KIP77_RS20195 (KIP77_20060)	10490..10633	-	144	WP_001125244.1	hypothetical protein	-
KIP77_RS20200 (KIP77_20065)	10653..11228	-	576	WP_001096616.1	plasmid replication DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11303	11303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T202703 WP_000438826.1 NZ_CP074712:c6692-6405 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T202703 NZ_CP099590:980115-980450 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12227.88 Da
• Isoelectric Point: 8.4628

>AT202703 WP_061855757.1 NZ_CP074712:c6418-6104 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT202703 NZ_CP099590:979867-980115 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: A0A858UV00