Detailed information of TA system TA202702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 26949..27537
• Replicon: plasmid unnamed5
• Accession: NZ_CP074711
• Organism: Acinetobacter baumannii strain ATCC 17978

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: KIP77_RS20065
• Protein ID: WP_000438826.1
• Coordinates: 26949..27236 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A858UV00
• Locus tag: KIP77_RS20070
• Protein ID: WP_061855757.1
• Coordinates: 27223..27537 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIP77_RS20035 (KIP77_19900)	22315..22704	+	390	WP_000078281.1	tautomerase family protein	-
KIP77_RS20040 (KIP77_19905)	22887..23030	-	144	WP_001125249.1	hypothetical protein	-
KIP77_RS20045 (KIP77_19910)	23051..23605	-	555	WP_001096621.1	plasmid replication DNA-binding protein	-
KIP77_RS20050 (KIP77_19915)	23598..24554	-	957	WP_000786840.1	replication initiation protein RepM	-
KIP77_RS20055 (KIP77_19920)	25519..25887	+	369	WP_000139283.1	LysR family transcriptional regulator	-
KIP77_RS20060 (KIP77_19925)	26086..26226	+	141	Protein_35	transposase	-
KIP77_RS20065 (KIP77_19930)	26949..27236	+	288	WP_000438826.1	BrnT family toxin	Toxin
KIP77_RS20070 (KIP77_19935)	27223..27537	+	315	WP_061855757.1	BrnA antitoxin family protein	Antitoxin
KIP77_RS20075 (KIP77_19940)	27666..30077	+	2412	WP_000932945.1	TonB-dependent receptor ZnuD2	-
KIP77_RS20080 (KIP77_19945)	30174..30872	+	699	WP_000007662.1	CPBP family glutamic-type intramembrane protease	-
KIP77_RS20085 (KIP77_19950)	30994..31191	-	198	WP_001282483.1	hypothetical protein	-
KIP77_RS20090 (KIP77_19955)	31191..31562	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38118	38118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T202702 WP_000438826.1 NZ_CP074711:26949-27236 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T202702 NZ_CP099590:850383-850790 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12227.88 Da
• Isoelectric Point: 8.4628

>AT202702 WP_061855757.1 NZ_CP074711:27223-27537 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT202702 NZ_CP099590:850136-850402 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: A0A858UV00