Detailed information of TA system TA202696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 12028..12616
• Replicon: plasmid unnamed3
• Accession: NZ_CP074707
• Organism: Acinetobacter baumannii strain ATCC 17978

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: KIP77_RS00080
• Protein ID: WP_000438826.1
• Coordinates: 12028..12315 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A858UV00
• Locus tag: KIP77_RS00085
• Protein ID: WP_061855757.1
• Coordinates: 12302..12616 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIP77_RS00045 (KIP77_00045)	7492..8067	+	576	WP_001096616.1	plasmid replication DNA-binding protein	-
KIP77_RS00050 (KIP77_00050)	8087..8230	+	144	WP_001125244.1	hypothetical protein	-
KIP77_RS00055 (KIP77_00055)	8415..8618	-	204	WP_000482656.1	hypothetical protein	-
KIP77_RS00060 (KIP77_00060)	8862..9107	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KIP77_RS00065 (KIP77_00065)	9097..9390	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KIP77_RS00070 (KIP77_00070)	9608..10810	+	1203	WP_061855756.1	alpha/beta fold hydrolase	-
KIP77_RS00075 (KIP77_00075)	10908..11681	+	774	WP_061855758.1	glucose 1-dehydrogenase	-
KIP77_RS00080 (KIP77_00080)	12028..12315	+	288	WP_000438826.1	BrnT family toxin	Toxin
KIP77_RS00085 (KIP77_00085)	12302..12616	+	315	WP_061855757.1	BrnA antitoxin family protein	Antitoxin
KIP77_RS00090 (KIP77_00090)	12745..15156	+	2412	WP_000932945.1	TonB-dependent receptor ZnuD2	-
KIP77_RS00095 (KIP77_00095)	15253..15951	+	699	WP_000007662.1	CPBP family glutamic-type intramembrane protease	-
KIP77_RS00100 (KIP77_00100)	16073..16270	-	198	WP_001282483.1	hypothetical protein	-
KIP77_RS00105 (KIP77_00105)	16270..16641	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..20351	20351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T202696 WP_000438826.1 NZ_CP074707:12028-12315 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T202696 NZ_CP099589:44416-44814 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGGTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12227.88 Da
• Isoelectric Point: 8.4628

>AT202696 WP_061855757.1 NZ_CP074707:12302-12616 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT202696 NZ_CP099589:44189-44416 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: A0A858UV00