Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 726..1314 Replicon plasmid unnamed3
Accession NZ_CP074707
Organism Acinetobacter baumannii strain ATCC 17978

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag KIP77_RS00010 Protein ID WP_000438826.1
Coordinates 726..1013 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A858UV00
Locus tag KIP77_RS00015 Protein ID WP_061855757.1
Coordinates 1000..1314 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIP77_RS00010 (KIP77_00010) 726..1013 + 288 WP_000438826.1 BrnT family toxin Toxin
KIP77_RS00015 (KIP77_00015) 1000..1314 + 315 WP_061855757.1 BrnA antitoxin family protein Antitoxin
KIP77_RS00020 (KIP77_00020) 1443..3854 + 2412 WP_000932945.1 TonB-dependent receptor ZnuD2 -
KIP77_RS00025 (KIP77_00025) 3951..4649 + 699 WP_000007662.1 CPBP family glutamic-type intramembrane protease -
KIP77_RS00030 (KIP77_00030) 4771..4968 - 198 WP_001282483.1 hypothetical protein -
KIP77_RS00035 (KIP77_00035) 4968..5339 - 372 WP_000504218.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..20351 20351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T202694 WP_000438826.1 NZ_CP074707:726-1013 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T202694 NZ_CP099588:c4205220-4205047 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12227.88 Da        Isoelectric Point: 8.4628

>AT202694 WP_061855757.1 NZ_CP074707:1000-1314 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT202694 NZ_CP099588:c4205544-4205248 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB A0A858UV00

References