Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 58758..59359 Replicon plasmid p702_18_3
Accession NZ_CP074704
Organism Escherichia coli strain 702/18

Toxin (Protein)


Gene name doc Uniprot ID A0A6G4LZ59
Locus tag KH969_RS26385 Protein ID WP_001216040.1
Coordinates 58979..59359 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KH969_RS26380 Protein ID WP_001190712.1
Coordinates 58758..58979 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH969_RS26335 (KH969_26345) 54270..54533 + 264 WP_000224227.1 hypothetical protein -
KH969_RS27405 54544..54669 + 126 Protein_69 hypothetical protein -
KH969_RS26345 (KH969_26355) 54923..55450 + 528 WP_097507128.1 DUF551 domain-containing protein -
KH969_RS26350 (KH969_26360) 55443..55664 + 222 WP_001683865.1 hypothetical protein -
KH969_RS26355 (KH969_26365) 55661..56224 + 564 WP_257232841.1 hypothetical protein -
KH969_RS26360 (KH969_26370) 56529..57053 + 525 WP_217477966.1 toprim domain-containing protein -
KH969_RS26365 (KH969_26375) 57180..57536 - 357 WP_001062547.1 hypothetical protein -
KH969_RS26370 (KH969_26380) 57537..58121 - 585 WP_139509859.1 DNA-binding protein -
KH969_RS26375 (KH969_26385) 58296..58685 + 390 WP_000506724.1 S24 family peptidase -
KH969_RS26380 (KH969_26390) 58758..58979 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KH969_RS26385 (KH969_26395) 58979..59359 + 381 WP_001216040.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KH969_RS26390 (KH969_26400) 59395..60489 - 1095 WP_000201932.1 hypothetical protein -
KH969_RS26395 (KH969_26405) 60479..61246 - 768 WP_000446290.1 hypothetical protein -
KH969_RS26400 (KH969_26410) 61253..61693 - 441 WP_001061875.1 DUF2829 domain-containing protein -
KH969_RS26405 (KH969_26415) 61708..62202 - 495 WP_000640907.1 dUTP diphosphatase -
KH969_RS26410 (KH969_26420) 62199..62675 - 477 WP_139509860.1 hypothetical protein -
KH969_RS26415 (KH969_26425) 62672..62980 - 309 WP_001350899.1 hypothetical protein -
KH969_RS26420 (KH969_26430) 63090..64118 - 1029 WP_001292231.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..95167 95167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13678.59 Da        Isoelectric Point: 6.2282

>T202691 WP_001216040.1 NZ_CP074704:58979-59359 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDDPVLVELAVGAATGEIPVSSVAKKLRELYGSNI

Download         Length: 381 bp

>T202691 NZ_CP099588:3978288-3978629 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT202691 WP_001190712.1 NZ_CP074704:58758-58979 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT202691 NZ_CP099588:c3978293-3978217 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G4LZ59

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References