Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94142..94785 Replicon plasmid p702_18_2
Accession NZ_CP074703
Organism Escherichia coli strain 702/18

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KH969_RS25890 Protein ID WP_001034044.1
Coordinates 94369..94785 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KH969_RS25885 Protein ID WP_001261286.1
Coordinates 94142..94372 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH969_RS25870 (KH969_25880) 89279..89509 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
KH969_RS25875 (KH969_25885) 89506..89922 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KH969_RS25880 (KH969_25890) 89967..93761 - 3795 WP_001144732.1 hypothetical protein -
KH969_RS25885 (KH969_25895) 94142..94372 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KH969_RS25890 (KH969_25900) 94369..94785 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KH969_RS25895 (KH969_25905) 94860..96425 + 1566 WP_001128474.1 AAA family ATPase -
KH969_RS25900 (KH969_25910) 96410..97432 + 1023 WP_000361402.1 helicase UvrD -
KH969_RS25910 (KH969_25920) 98739..99653 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / dfrA17 / floR / aac(3)-IIa / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-Ia / aac(3)-IId / sitABCD iucA / iucB / iucC / iucD / iutA 1..116496 116496
- flank IS/Tn sitABCD - 97686..102188 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T202689 WP_001034044.1 NZ_CP074703:94369-94785 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T202689 NZ_CP099588:c3664175-3663777 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT202689 WP_001261286.1 NZ_CP074703:94142-94372 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT202689 NZ_CP099588:c3664471-3664178 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References