Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 89279..89922 Replicon plasmid p702_18_2
Accession NZ_CP074703
Organism Escherichia coli strain 702/18

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KH969_RS25875 Protein ID WP_001034046.1
Coordinates 89506..89922 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KH969_RS25870 Protein ID WP_001261278.1
Coordinates 89279..89509 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH969_RS25840 (KH969_25850) 84790..85095 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KH969_RS25845 (KH969_25855) 85097..85315 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KH969_RS25850 (KH969_25860) 85906..86394 + 489 WP_011254646.1 hypothetical protein -
KH969_RS25855 (KH969_25865) 86428..87561 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
KH969_RS25860 (KH969_25870) 87728..88501 - 774 WP_000905949.1 hypothetical protein -
KH969_RS25865 (KH969_25875) 88514..89014 - 501 WP_000528931.1 HEPN family nuclease -
KH969_RS25870 (KH969_25880) 89279..89509 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KH969_RS25875 (KH969_25885) 89506..89922 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KH969_RS25880 (KH969_25890) 89967..93761 - 3795 WP_001144732.1 hypothetical protein -
KH969_RS25885 (KH969_25895) 94142..94372 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KH969_RS25890 (KH969_25900) 94369..94785 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / dfrA17 / floR / aac(3)-IIa / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-Ia / aac(3)-IId / sitABCD iucA / iucB / iucC / iucD / iutA 1..116496 116496


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T202688 WP_001034046.1 NZ_CP074703:89506-89922 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T202688 NZ_CP099588:3653583-3653924 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT202688 WP_001261278.1 NZ_CP074703:89279-89509 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT202688 NZ_CP099588:3653245-3653562 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References