Detailed information of TA system TA202687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 84790..85315
• Replicon: plasmid p702_18_2
• Accession: NZ_CP074703
• Organism: Escherichia coli strain 702/18

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: KH969_RS25840
• Protein ID: WP_001159868.1
• Coordinates: 84790..85095 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: KH969_RS25845
• Protein ID: WP_000813634.1
• Coordinates: 85097..85315 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH969_RS25825 (KH969_25835)	80700..81866	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
KH969_RS25830 (KH969_25840)	82454..83209	-	756	WP_000852146.1	replication initiation protein RepE	-
KH969_RS25835 (KH969_25845)	83983..84789	-	807	WP_000016982.1	site-specific integrase	-
KH969_RS25840 (KH969_25850)	84790..85095	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
KH969_RS25845 (KH969_25855)	85097..85315	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KH969_RS25850 (KH969_25860)	85906..86394	+	489	WP_011254646.1	hypothetical protein	-
KH969_RS25855 (KH969_25865)	86428..87561	-	1134	WP_001542067.1	DUF3800 domain-containing protein	-
KH969_RS25860 (KH969_25870)	87728..88501	-	774	WP_000905949.1	hypothetical protein	-
KH969_RS25865 (KH969_25875)	88514..89014	-	501	WP_000528931.1	HEPN family nuclease	-
KH969_RS25870 (KH969_25880)	89279..89509	+	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
KH969_RS25875 (KH969_25885)	89506..89922	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / dfrA17 / floR / aac(3)-IIa / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-Ia / aac(3)-IId / sitABCD	iucA / iucB / iucC / iucD / iutA	1..116496	116496

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T202687 WP_001159868.1 NZ_CP074703:c85095-84790 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T202687 NZ_CP099588:3436944-3437162 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT202687 WP_000813634.1 NZ_CP074703:c85315-85097 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT202687 NZ_CP099588:3436544-3436918 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2