Detailed information of TA system TA202685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 85474..87097
• Replicon: plasmid p702_18_1
• Accession: NZ_CP074702
• Organism: Escherichia coli strain 702/18

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: KH969_RS24610
• Protein ID: WP_000088046.1
• Coordinates: 85772..87097 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: KH969_RS24605
• Protein ID: WP_024136327.1
• Coordinates: 85474..85767 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH969_RS24580 (KH969_24590)	81290..82531	+	1242	WP_001053338.1	VWA domain-containing protein	-
KH969_RS27335	82622..82753	+	132	WP_000374059.1	hypothetical protein	-
KH969_RS24585 (KH969_24595)	83094..83375	-	282	WP_000077926.1	hypothetical protein	-
KH969_RS24590 (KH969_24600)	83425..83616	-	192	WP_000398480.1	hypothetical protein	-
KH969_RS24595 (KH969_24605)	83708..84049	-	342	WP_001151575.1	hypothetical protein	-
KH969_RS24600 (KH969_24610)	84422..84814	+	393	WP_000341066.1	hypothetical protein	-
KH969_RS24605 (KH969_24615)	85474..85767	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
KH969_RS24610 (KH969_24620)	85772..87097	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
KH969_RS24615 (KH969_24625)	87158..87364	+	207	WP_000134171.1	hypothetical protein	-
KH969_RS24620 (KH969_24630)	87466..87876	+	411	WP_000985911.1	hypothetical protein	-
KH969_RS24625 (KH969_24635)	87889..88704	+	816	WP_053276223.1	HNH endonuclease signature motif containing protein	-
KH969_RS24630 (KH969_24640)	88938..89635	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
KH969_RS24635 (KH969_24645)	89679..90344	-	666	WP_000490638.1	hypothetical protein	-
KH969_RS24640 (KH969_24650)	90402..90782	-	381	WP_001426317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / lnu(F) / blaCTX-M-14b	-	1..219118	219118
	-	flank	IS/Tn	-	-	88938..89213	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T202685 WP_000088046.1 NZ_CP074702:85772-87097 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T202685 NZ_CP099588:2599246-2599353 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT202685 WP_024136327.1 NZ_CP074702:85474-85767 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT202685 NZ_CP099588:c2599199-2599133 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1