Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4515825..4516443 Replicon chromosome
Accession NZ_CP074701
Organism Escherichia coli strain 702/18

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KH969_RS21530 Protein ID WP_001291435.1
Coordinates 4515825..4516043 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KH969_RS21535 Protein ID WP_000344800.1
Coordinates 4516069..4516443 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH969_RS21495 (4511115) 4511115..4511687 + 573 WP_000779831.1 YbaY family lipoprotein -
KH969_RS21500 (4511718) 4511718..4512029 - 312 WP_000409911.1 MGMT family protein -
KH969_RS21510 (4512408) 4512408..4512761 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KH969_RS21515 (4512802) 4512802..4514352 - 1551 WP_012311818.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KH969_RS21520 (4514516) 4514516..4514986 - 471 WP_000136192.1 YlaC family protein -
KH969_RS21525 (4515102) 4515102..4515653 - 552 WP_000102561.1 maltose O-acetyltransferase -
KH969_RS21530 (4515825) 4515825..4516043 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KH969_RS21535 (4516069) 4516069..4516443 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KH969_RS21540 (4516989) 4516989..4520138 - 3150 WP_072754545.1 efflux RND transporter permease AcrB -
KH969_RS21545 (4520161) 4520161..4521354 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202683 WP_001291435.1 NZ_CP074701:c4516043-4515825 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T202683 NZ_CP099588:c2359633-2359457 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT202683 WP_000344800.1 NZ_CP074701:c4516443-4516069 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT202683 NZ_CP099588:c2359411-2358995 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References