202682

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4481517..4482354 Replicon chromosome
Accession NZ_CP074701
Organism Escherichia coli strain 702/18

Toxin (Protein)


Gene name itaT Uniprot ID V0SED1
Locus tag KH969_RS21360 Protein ID WP_000227787.1
Coordinates 4481517..4482059 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag KH969_RS21365 Protein ID WP_001353405.1
Coordinates 4482043..4482354 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH969_RS21340 (4477057) 4477057..4477968 - 912 WP_000705871.1 2-dehydropantoate 2-reductase -
KH969_RS21345 (4478136) 4478136..4478627 + 492 WP_001138904.1 nucleotide binding protein YajQ -
KH969_RS21350 (4478755) 4478755..4480119 - 1365 WP_001000978.1 MFS transporter -
KH969_RS21355 (4480526) 4480526..4481461 + 936 WP_012311701.1 tetratricopeptide repeat protein -
KH969_RS21360 (4481517) 4481517..4482059 - 543 WP_000227787.1 GNAT family N-acetyltransferase Toxin
KH969_RS21365 (4482043) 4482043..4482354 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
KH969_RS21370 (4482539) 4482539..4483429 - 891 WP_000971336.1 heme o synthase -
KH969_RS21375 (4483441) 4483441..4483770 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
KH969_RS21380 (4483770) 4483770..4484384 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
KH969_RS21385 (4484374) 4484374..4486365 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
KH969_RS21390 (4486387) 4486387..4487334 - 948 WP_001239436.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19946.24 Da        Isoelectric Point: 8.8951

>T202682 WP_000227787.1 NZ_CP074701:c4482059-4481517 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T202682 NZ_CP099588:2276743-2278065 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT202682 WP_001353405.1 NZ_CP074701:c4482354-4482043 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT202682 NZ_CP099588:2276477-2276743 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GE43

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References