Detailed information of TA system TA202680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3795520..3796115
• Replicon: chromosome
• Accession: NZ_CP074701
• Organism: Escherichia coli strain 702/18

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: KH969_RS18220
• Protein ID: WP_000239581.1
• Coordinates: 3795765..3796115 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: KH969_RS18215
• Protein ID: WP_001223213.1
• Coordinates: 3795520..3795771 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH969_RS18205 (3791185)	3791185..3794964	+	3780	WP_000060872.1	autotransporter assembly complex protein TamB	-
KH969_RS18210 (3794967)	3794967..3795308	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
KH969_RS18215 (3795520)	3795520..3795771	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
KH969_RS18220 (3795765)	3795765..3796115	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
KH969_RS18225 (3796194)	3796194..3796724	-	531	WP_000055075.1	inorganic diphosphatase	-
KH969_RS18230 (3797034)	3797034..3797990	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
KH969_RS18235 (3798123)	3798123..3799625	+	1503	WP_001095664.1	sugar ABC transporter ATP-binding protein	-
KH969_RS18240 (3799639)	3799639..3800661	+	1023	WP_012311695.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T202680 WP_000239581.1 NZ_CP074701:3795765-3796115 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T202680 NZ_CP074701:3795765-3796115 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT202680 WP_001223213.1 NZ_CP074701:3795520-3795771 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT202680 NZ_CP074701:3795520-3795771 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7