20268

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 74325..74884 Replicon plasmid pO157
Accession NC_002128
Organism Escherichia coli O157:H7 str. Sakai

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag pO157p70 Protein ID NP_052677.1
Coordinates 74609..74884 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID O82927
Locus tag pO157p69 Protein ID NP_052676.1
Coordinates 74325..74609 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
pO157p64 69517..70077 + 561 NP_052671.1 conjugal transfer fertility inhibition protein FinO -
ECp070 70210..70422 + 213 NP_958725.1 hypothetical protein -
pO157p65 70667..71128 + 462 NP_052672.1 hypothetical protein -
ECp072 71174..71383 + 210 NP_958726.1 hypothetical protein -
pO157p66 71421..71759 + 339 NP_052673.1 hypothetical protein -
pO157p67 71999..72253 + 255 NP_052674.1 replication protein -
pO157p68 72556..73413 + 858 NP_052675.1 replication protein -
pO157p69 74325..74609 + 285 NP_052676.1 hypothetical protein Antitoxin
pO157p70 74609..74884 + 276 NP_052677.1 hypothetical protein Toxin
pO157p71 74979..75185 + 207 NP_052678.1 hypothetical protein -
pO157p74 76062..76526 - 465 NP_052681.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..92721 92721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T20268 NP_052677.1 NC_002128:74609-74884 [Escherichia coli O157:H7 str. Sakai]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T20268 NC_002128:74609-74884 [Escherichia coli O157:H7 str. Sakai]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT20268 NP_052676.1 NC_002128:74325-74609 [Escherichia coli O157:H7 str. Sakai]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT20268 NC_002128:74325-74609 [Escherichia coli O157:H7 str. Sakai]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB O82927

References