Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1099066..1099850 Replicon chromosome
Accession NZ_CP074701
Organism Escherichia coli strain 702/18

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag KH969_RS05440 Protein ID WP_000613626.1
Coordinates 1099356..1099850 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag KH969_RS05435 Protein ID WP_001110446.1
Coordinates 1099066..1099359 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH969_RS05425 (1094227) 1094227..1095186 - 960 WP_000846337.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
KH969_RS05430 (1095759) 1095759..1098932 + 3174 WP_023141578.1 ribonuclease E -
KH969_RS05435 (1099066) 1099066..1099359 + 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
KH969_RS05440 (1099356) 1099356..1099850 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
KH969_RS05445 (1099945) 1099945..1100898 - 954 WP_001212763.1 flagellar hook-associated protein FlgL -
KH969_RS05450 (1100910) 1100910..1102553 - 1644 WP_000096448.1 flagellar hook-associated protein FlgK -
KH969_RS05455 (1102619) 1102619..1103560 - 942 WP_012311956.1 flagellar assembly peptidoglycan hydrolase FlgJ -
KH969_RS05460 (1103560) 1103560..1104657 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T202674 WP_000613626.1 NZ_CP074701:1099356-1099850 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T202674 NZ_CP099588:974534-974869 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT202674 WP_001110446.1 NZ_CP074701:1099066-1099359 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT202674 NZ_CP099588:974286-974534 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References