Detailed information of TA system TA202673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 780393..781031
• Replicon: chromosome
• Accession: NZ_CP074701
• Organism: Escherichia coli strain 702/18

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KH969_RS03895
• Protein ID: WP_000813794.1
• Coordinates: 780855..781031 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KH969_RS03890
• Protein ID: WP_001270286.1
• Coordinates: 780393..780809 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH969_RS03870 (775545)	775545..776486	-	942	WP_001251303.1	ABC transporter permease	-
KH969_RS03875 (776487)	776487..777500	-	1014	WP_000220391.1	ABC transporter ATP-binding protein	-
KH969_RS03880 (777518)	777518..778663	-	1146	WP_000034364.1	ABC transporter substrate-binding protein	-
KH969_RS03885 (778908)	778908..780314	-	1407	WP_000760611.1	PLP-dependent aminotransferase family protein	-
KH969_RS03890 (780393)	780393..780809	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KH969_RS03895 (780855)	780855..781031	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KH969_RS03900 (781253)	781253..781483	+	231	WP_000491567.1	DUF2554 family protein	-
KH969_RS03905 (781575)	781575..783536	-	1962	WP_012311648.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KH969_RS03910 (783609)	783609..784145	-	537	WP_000429154.1	DNA-binding transcriptional regulator SutR	-
KH969_RS03915 (784198)	784198..785118	+	921	Protein_777	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	785133..786461	1328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T202673 WP_000813794.1 NZ_CP074701:c781031-780855 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T202673 NZ_CP099588:843603-844010 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT202673 WP_001270286.1 NZ_CP074701:c780809-780393 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT202673 NZ_CP099588:843356-843622 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6HPB