20267

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 28682..29207 Replicon plasmid pO157
Accession NC_002128
Organism Escherichia coli O157:H7 str. Sakai

Toxin (Protein)


Gene name ccdB Uniprot ID P62555
Locus tag pO157p29 Protein ID NP_052635.1
Coordinates 28902..29207 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID P62553
Locus tag pO157p28 Protein ID NP_052634.1
Coordinates 28682..28900 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECp023 23965..24195 + 231 NP_958721.1 hypothetical protein -
pO157p22 24316..25056 + 741 NP_052628.1 recombinase -
pO157p23 24745..25062 - 318 NP_052629.1 hypothetical protein -
pO157p24 25341..26318 - 978 NP_052630.2 RepFIB -
pO157p25 26056..26385 + 330 NP_052631.1 hypothetical protein -
ECp025.2n 26726..26926 - 201 NP_958722.1 hypothetical protein -
pO157p26 26923..27543 - 621 NP_052632.1 hypothetical protein -
pO157p27 27540..28223 - 684 NP_052633.1 KfrAs -
pO157p28 28682..28900 + 219 NP_052634.1 plasmid maintenance protein CcdA Antitoxin
pO157p29 28902..29207 + 306 NP_052635.1 plasmid maintenance protein CcdB Toxin
pO157p30 29208..30014 + 807 NP_052636.1 resolvase -
pO157p31 30736..31626 - 891 NP_052637.1 transposase -
pO157p83 31623..31949 - 327 NP_052638.1 hypothetical protein -
pO157p33 32837..34003 + 1167 NP_052640.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..92721 92721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T20267 NP_052635.1 NC_002128:28902-29207 [Escherichia coli O157:H7 str. Sakai]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T20267 NC_002128:28902-29207 [Escherichia coli O157:H7 str. Sakai]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT20267 NP_052634.1 NC_002128:28682-28900 [Escherichia coli O157:H7 str. Sakai]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT20267 NC_002128:28682-28900 [Escherichia coli O157:H7 str. Sakai]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAV1

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z6FAV4

References