Detailed information of TA system TA020266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/VapC(toxin)
• Location: 418347..418981
• Replicon: chromosome
• Accession: NC_001263
• Organism: Deinococcus radiodurans R1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q9RX96
• Locus tag: DR_RS02160
• Protein ID: WP_010887064.1
• Coordinates: 418568..418981 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DR_RS02155
• Protein ID: WP_162177573.1
• Coordinates: 418347..418571 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR_RS02120 (DR_0411)	413498..414181	+	684	WP_010887056.1	SDR family oxidoreductase	-
DR_RS02125 (DR_0412)	414202..415164	+	963	WP_034349507.1	NAD(P)/FAD-dependent oxidoreductase	-
DR_RS02130 (DR_0413)	415187..415366	+	180	WP_034349506.1	hypothetical protein	-
DR_RS02135 (DR_0414)	415368..415996	+	629	Protein_407	HAD family hydrolase	-
DR_RS02140 (DR_0415)	416044..417525	-	1482	Protein_408	carboxylesterase/lipase family protein	-
DR_RS02145 (DR_0416)	417705..417947	+	243	WP_010887061.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
DR_RS02150 (DR_0417)	417941..418294	+	354	WP_010887062.1	endoribonuclease MazF	-
DR_RS02155 (DR_0418)	418347..418571	+	225	WP_162177573.1	hypothetical protein	Antitoxin
DR_RS02160 (DR_0419)	418568..418981	+	414	WP_010887064.1	type II toxin-antitoxin system VapC family toxin	Toxin
DR_RS02165 (DR_0420)	418978..421446	+	2469	WP_010887065.1	ATP-dependent helicase HrpB	-
DR_RS02170 (DR_0421)	421627..421851	-	225	WP_162177572.1	hypothetical protein	-
DR_RS02175 (DR_0422)	422007..422783	-	777	WP_010887067.1	trans-aconitate 2-methyltransferase	-
DR_RS02180	422904..423083	-	180	WP_028328008.1	hypothetical protein	-
DR_RS02185 (DR_0423)	423080..423706	-	627	WP_010887068.1	single-stranded DNA-binding protein DdrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14634.68 Da
• Isoelectric Point: 5.5820

>T20266 WP_010887064.1 NC_001263:418568-418981 [Deinococcus radiodurans R1]
MTESTVLDANILSALLRSEANPLSIARQLSALAREGSLVVYAELLAGPEVTPDFLKGFLQEVDVQILPPSGLAVWESAGL
AYGSYAQRRQRSGGGSPRQILSDFVIGAHALYHGAALFTADPQHYRLSFPALTVLTP

Nucleotide

• Length: 414 bp

>T20266 NC_001263:418568-418981 [Deinococcus radiodurans R1]
ATGACAGAGAGCACCGTGCTGGACGCCAATATTCTCAGTGCCCTGCTCCGTTCTGAGGCGAACCCTCTGTCCATTGCGCG
TCAGCTTTCGGCGCTGGCCCGTGAGGGTTCACTAGTGGTTTATGCCGAGTTGCTCGCGGGCCCTGAGGTTACGCCCGATT
TCCTCAAGGGATTTCTGCAAGAGGTGGACGTGCAGATTCTTCCGCCGTCTGGCCTTGCGGTCTGGGAAAGCGCGGGCCTC
GCTTATGGGAGCTACGCTCAGCGGCGGCAACGCAGCGGTGGTGGGTCGCCTCGCCAGATTCTGAGTGACTTTGTGATTGG
TGCCCATGCCCTGTATCACGGCGCCGCTCTCTTCACCGCTGATCCTCAGCATTACCGCCTCAGCTTCCCAGCCCTCACAG
TCCTGACCCCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8229.23 Da
• Isoelectric Point: 4.3481

>AT20266 WP_162177573.1 NC_001263:418347-418571 [Deinococcus radiodurans R1]
MTTLEARIENGKVVLTPEQAAELGLREGEVLPVTIAPHEAETEGNPFTRWIGTLPPLPDGEESARFYRRLRDGE

Nucleotide

• Length: 225 bp

>AT20266 NC_001263:418347-418571 [Deinococcus radiodurans R1]
ATGACGACGTTGGAGGCCCGGATTGAGAACGGCAAGGTGGTGCTCACCCCTGAGCAGGCCGCCGAACTGGGCCTGCGCGA
AGGCGAAGTGCTGCCGGTGACGATTGCGCCGCACGAAGCGGAAACGGAAGGCAACCCCTTCACCCGCTGGATTGGGACGC
TCCCCCCACTGCCTGACGGTGAAGAGTCTGCGCGGTTCTACCGCCGCCTGCGAGACGGCGAATGA

Structures

Toxin

• AlphaFold DB: Q9RX96