Detailed information of TA system TA202655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1258120..1259037
• Replicon: chromosome
• Accession: NZ_CP074685
• Organism: Bacillus velezensis strain VTX9

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6A8LGT8
• Locus tag: KH263_RS06370
• Protein ID: WP_003154806.1
• Coordinates: 1258291..1259037 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KH263_RS06365
• Protein ID: WP_003154807.1
• Coordinates: 1258120..1258290 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH263_RS06330 (KH263_06330)	1253351..1254973	+	1623	WP_003154821.1	pyocin knob domain-containing protein	-
KH263_RS06335 (KH263_06335)	1254986..1255357	+	372	WP_014304856.1	XkdW family protein	-
KH263_RS06340 (KH263_06340)	1255363..1255560	+	198	WP_044053582.1	XkdX family protein	-
KH263_RS06345 (KH263_06345)	1255617..1256378	+	762	WP_033575307.1	hypothetical protein	-
KH263_RS06350 (KH263_06350)	1256430..1256693	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KH263_RS06355 (KH263_06355)	1256707..1256970	+	264	WP_003154813.1	phage holin	-
KH263_RS06360 (KH263_06360)	1256984..1257862	+	879	WP_014304858.1	N-acetylmuramoyl-L-alanine amidase	-
KH263_RS19940	1257898..1258023	-	126	WP_003154809.1	hypothetical protein	-
KH263_RS06365 (KH263_06365)	1258120..1258290	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KH263_RS06370 (KH263_06370)	1258291..1259037	-	747	WP_003154806.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KH263_RS06375 (KH263_06375)	1259142..1260140	-	999	WP_003154805.1	inorganic phosphate transporter	-
KH263_RS06380 (KH263_06380)	1260153..1260770	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KH263_RS06385 (KH263_06385)	1261056..1262372	-	1317	WP_046559522.1	amino acid permease	-
KH263_RS06390 (KH263_06390)	1262695..1263645	+	951	WP_060386897.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29034.50 Da
• Isoelectric Point: 4.6947

>T202655 WP_003154806.1 NZ_CP074685:c1259037-1258291 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T202655 NZ_CP099576:c888565-888377 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT202655 WP_003154807.1 NZ_CP074685:c1258290-1258120 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT202655 NZ_CP099576:c888854-888594 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A6A8LGT8

Antitoxin

• AlphaFold DB: I2HQ14