Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 135937..136580 Replicon plasmid pMEI008_1
Accession NZ_CP074681
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KIM92_RS24260 Protein ID WP_001034044.1
Coordinates 136164..136580 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KIM92_RS24255 Protein ID WP_001261286.1
Coordinates 135937..136167 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS24240 (131074) 131074..131304 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
KIM92_RS24245 (131301) 131301..131717 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KIM92_RS24250 (131762) 131762..135556 - 3795 WP_001144731.1 hypothetical protein -
KIM92_RS24255 (135937) 135937..136167 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KIM92_RS24260 (136164) 136164..136580 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KIM92_RS24265 (136655) 136655..138220 + 1566 WP_001128474.1 AAA family ATPase -
KIM92_RS24270 (138205) 138205..139227 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..158291 158291
- flank IS/Tn - - 139481..139984 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T202653 WP_001034044.1 NZ_CP074681:136164-136580 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T202653 NZ_CP099575:5239876-5240169 [Pseudomonas kermanshahensis]
ATGACTGAAACATTTTTTGAGTTCGATGCGGCCGATTACCTGACCACTCCGCAAGCCATCGCTCAATTCATGTCCGATGC
GCTGGAAACGGGTGATGCCAGCTACGTGGCCAAAGCCGTTGGCGTAGTGGCTCGTGCAAAAGGGATGAGCGATCTGGCGA
AAGAAACCGGCTTGTCTCGTGAGCAACTTTACCGATCGTTCAGCGAACACGGCAATCCGACCCTTAAATCGTTCCTCGCA
GTCATGAAGGCGTTGGGCATAGACATGACGGCAAAACCTCACCTTGCACACTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT202653 WP_001261286.1 NZ_CP074681:135937-136167 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT202653 NZ_CP099575:5239581-5239883 [Pseudomonas kermanshahensis]
ATGAAGAAGATCGAATCCAGCAGTTTCAGACATTGGGTGCATGGGCTCCGGGACGAAATGGCGCGTATCCGGATCATTGC
TCGTATTAACCGTTTGATGGAGGGCCTGTCTGGTGACGTCTCAGCAGTTGGCCACGGCGTGAGTGAACTCCGAATTCACC
ATGGTCCGGGCTACCGGGTGTACTTTCACCAAGCAGGCGATACGTTGGTAATACTGCTTTGCGGCGGGGACAAGAGCAGC
CAGCGGCGGGACATCAAAGCGGCTCACGAAATCCTGCGTTCCTGGAGGATGCAAAATGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References