Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 131074..131717 Replicon plasmid pMEI008_1
Accession NZ_CP074681
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KIM92_RS24245 Protein ID WP_001034046.1
Coordinates 131301..131717 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KIM92_RS24240 Protein ID WP_001261278.1
Coordinates 131074..131304 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS24210 (126584) 126584..126889 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KIM92_RS24215 (126891) 126891..127109 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KIM92_RS24220 (127701) 127701..128189 + 489 WP_011254646.1 hypothetical protein -
KIM92_RS24225 (128223) 128223..129356 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
KIM92_RS24230 (129523) 129523..130296 - 774 WP_000905949.1 hypothetical protein -
KIM92_RS24235 (130309) 130309..130809 - 501 WP_000528932.1 HEPN family nuclease -
KIM92_RS24240 (131074) 131074..131304 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KIM92_RS24245 (131301) 131301..131717 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KIM92_RS24250 (131762) 131762..135556 - 3795 WP_001144731.1 hypothetical protein -
KIM92_RS24255 (135937) 135937..136167 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KIM92_RS24260 (136164) 136164..136580 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..158291 158291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T202652 WP_001034046.1 NZ_CP074681:131301-131717 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T202652 NZ_CP099575:5239581-5239883 [Pseudomonas kermanshahensis]
ATGAAGAAGATCGAATCCAGCAGTTTCAGACATTGGGTGCATGGGCTCCGGGACGAAATGGCGCGTATCCGGATCATTGC
TCGTATTAACCGTTTGATGGAGGGCCTGTCTGGTGACGTCTCAGCAGTTGGCCACGGCGTGAGTGAACTCCGAATTCACC
ATGGTCCGGGCTACCGGGTGTACTTTCACCAAGCAGGCGATACGTTGGTAATACTGCTTTGCGGCGGGGACAAGAGCAGC
CAGCGGCGGGACATCAAAGCGGCTCACGAAATCCTGCGTTCCTGGAGGATGCAAAATGACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT202652 WP_001261278.1 NZ_CP074681:131074-131304 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT202652 NZ_CP099575:5239876-5240169 [Pseudomonas kermanshahensis]
ATGACTGAAACATTTTTTGAGTTCGATGCGGCCGATTACCTGACCACTCCGCAAGCCATCGCTCAATTCATGTCCGATGC
GCTGGAAACGGGTGATGCCAGCTACGTGGCCAAAGCCGTTGGCGTAGTGGCTCGTGCAAAAGGGATGAGCGATCTGGCGA
AAGAAACCGGCTTGTCTCGTGAGCAACTTTACCGATCGTTCAGCGAACACGGCAATCCGACCCTTAAATCGTTCCTCGCA
GTCATGAAGGCGTTGGGCATAGACATGACGGCAAAACCTCACCTTGCACACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References