Detailed information of TA system TA202651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 126584..127109
• Replicon: plasmid pMEI008_1
• Accession: NZ_CP074681
• Organism: Escherichia coli strain MEI008

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: KIM92_RS24210
• Protein ID: WP_001159868.1
• Coordinates: 126584..126889 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: KIM92_RS24215
• Protein ID: WP_000813634.1
• Coordinates: 126891..127109 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM92_RS24195 (122516)	122516..123682	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
KIM92_RS24200 (124270)	124270..125025	-	756	WP_000852146.1	replication initiation protein RepE	-
KIM92_RS24205 (125777)	125777..126583	-	807	WP_000016982.1	site-specific integrase	-
KIM92_RS24210 (126584)	126584..126889	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
KIM92_RS24215 (126891)	126891..127109	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KIM92_RS24220 (127701)	127701..128189	+	489	WP_011254646.1	hypothetical protein	-
KIM92_RS24225 (128223)	128223..129356	-	1134	WP_000545984.1	DUF3800 domain-containing protein	-
KIM92_RS24230 (129523)	129523..130296	-	774	WP_000905949.1	hypothetical protein	-
KIM92_RS24235 (130309)	130309..130809	-	501	WP_000528932.1	HEPN family nuclease	-
KIM92_RS24240 (131074)	131074..131304	+	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
KIM92_RS24245 (131301)	131301..131717	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..158291	158291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T202651 WP_001159868.1 NZ_CP074681:c126889-126584 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T202651 NZ_CP099575:c4962550-4962254 [Pseudomonas kermanshahensis]
ATGGAAAAGAGAACGCCTCATTGCCCACTCGATCGGGTGCGAGTGCTGTTGAGCCAGGGCAAGGTCAATACAACACAATC
GGCGACGCAGGGAGCGCTGGCGTTGGGTTTAGGGCCCTTTGACATCGTCGACATCGTTCGTGGCTTGTCCGTCAAGGATT
TCTATAAAAGCATGACGACCTACCATGATCACCGCCTTTGGCAAGATGTCTATCGCCCCCAAACTACTGTTGGTAGGGTC
TATCTCAAGTTAATGGTGATTGATGATCTGCTGATCGTGTCATTCAAGGAGCTTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT202651 WP_000813634.1 NZ_CP074681:c127109-126891 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT202651 NZ_CP099575:c4962252-4961851 [Pseudomonas kermanshahensis]
ATGAAATGCCCGATGTGTGGAGGAGCGGAGCTAGTCGCACAGGCGCATGACATGCCATACCAGTATAAAGGTGAGGCAAC
TGTAATTCCCGATGTCTTCGGCGATTACTGCGCTGCATGCGGCGAGAGTGTGCTGAGTATGGATGAAGCTCAGCGCATCA
GTGACCTGATGTCGGCGTTCAATCGCCAGGTCAACGCTGCGGCGGTCGACCCGGGTTTCATCGTCTCAGTGCGCAAGAAA
TTCGATCTCGACCAGCGTGAAGCAGGGGAGATCTTCGGGGGTGGCGTCAATGCGTTCTCCCGTTACGAAAACGGTAAAAC
CAGGCCACCCGTGGCGTTGGTCAAACTGTTCAAACTGCTGGATCGCCACCCGGAACTGTTCGAAGAAGTACGCACTGCCT
GA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2