Detailed information of TA system TA202647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 81114..81739
• Replicon: plasmid pMEI008_1
• Accession: NZ_CP074681
• Organism: Escherichia coli strain MEI008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KIM92_RS23915
• Protein ID: WP_000911313.1
• Coordinates: 81341..81739 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: KIM92_RS23910
• Protein ID: WP_000450520.1
• Coordinates: 81114..81341 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM92_RS23910 (81114)	81114..81341	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KIM92_RS23915 (81341)	81341..81739	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
KIM92_RS23920 (81748)	81748..83901	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
KIM92_RS23925 (84154)	84154..84864	-	711	WP_230133085.1	conjugal transfer complement resistance protein TraT	-
KIM92_RS23930 (84917)	84917..85414	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..158291	158291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T202647 WP_000911313.1 NZ_CP074681:81341-81739 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T202647 NZ_CP099551:1886150-1886497 [Lactiplantibacillus pentosus]
ATGACTTATTTGCCTAAGCAAAAAGATATCATCTGGATTGATTTTGATCCGCAACGCGGCCGCGAAATAAAAAAGCGCCG
TCCTGCTGTAATCCTTAGTAGTAACCTCTACACCCAGAACACCGGCTTTGTAATTGTTTCACCAATTACGTCTACCATTC
ATGATTTGCCCGGCTACTTTAGTCTCAATGGCTACGATATCCATGGACAAGTTGTCGCTGCACAGATTTATTCGTTTGAC
GCGAGGCCAAAAGCGGGGCGAAATATCACATATATCGAAACCATGCGCAACGTAGATTTTTATCGCGTCGCGCAGACAGT
GTACTACAACTTTGACTTTCCATTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT202647 WP_000450520.1 NZ_CP074681:81114-81341 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT202647 NZ_CP099551:1885932-1886150 [Lactiplantibacillus pentosus]
ATGATTGAAGTAAAAACAGTTAGCCGTGGCAACTCATTGGCATTAAGTCTGCCTAAAGACGGCCGTTTTAAAAAAGGCCA
ACGCTGGTTACTCATCCCATCAAAAGATGGGGAGTCATATACACTCGTACCACGTATTGAAAATCCTTATGCTGGTCCCA
AGTCAAAACAGCCAATGACTGAAGCGTGGTCTGATGTTAACTGGAATGAGGTCGAATAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2