Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4394975..4395472 Replicon chromosome
Accession NZ_CP074680
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KIM92_RS21320 Protein ID WP_001173343.1
Coordinates 4394975..4395148 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KIM92_RS21325 Protein ID WP_000398619.1
Coordinates 4395176..4395472 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS21310 (4391645) 4391645..4393102 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KIM92_RS21315 (4393339) 4393339..4394856 + 1518 WP_001295074.1 lysine--tRNA ligase -
KIM92_RS21320 (4394975) 4394975..4395148 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KIM92_RS21325 (4395176) 4395176..4395472 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KIM92_RS21330 (4395699) 4395699..4395971 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KIM92_RS21335 (4395983) 4395983..4396213 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
KIM92_RS21340 (4396394) 4396394..4398025 + 1632 WP_001216461.1 sensor histidine kinase -
KIM92_RS21345 (4398022) 4398022..4398741 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T202643 WP_001173343.1 NZ_CP074680:c4395148-4394975 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T202643 NZ_CP099544:755407-755667 [Photobacterium damselae]
ATGACCGTTTTGAGTTTTTCTGAAGTTCGAGGCAATTTTAAAAAAGTATGCGATCAAGTCGTTGATGATTGCGATGCAGT
AGTTATCCATCGACGTGATGCAGATAATGTCGTGCTTATGTCTGAATCTGAGTTTAATAGTTGGAAGGAAACGATGTATC
TCATGTCTAATCCTGTAAATGCTGCAAACTTACTTGAATCAATAAAACAAGCTGAATGTGGTAATGTACAGACACATGAG
CTACTAAGCCATGATGATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT202643 WP_000398619.1 NZ_CP074680:c4395472-4395176 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT202643 NZ_CP099544:755657-755917 [Photobacterium damselae]
ATGATGATTAATTTCACGCCTAACGCTTGGGATGATTACTTATACTGGCAATCTCAAGATAAGAAGACTTTAAAACGAAT
CAATAAACTGATTGAGAATTGTGTTAGAACGCCGTTCGAAGGAATTGGAAAGCCTGAACCATTGAAAGATAACTTATCAG
GCTATTGGTCGCGACGCATTGATGATACACATAGATTAGTCTATAAAGCCACTCAAGATACTTTAGTCATTGTTGCTGTT
CGGTTTCACTACCAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References