Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4292468..4293063 Replicon chromosome
Accession NZ_CP074680
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag KIM92_RS20805 Protein ID WP_000239579.1
Coordinates 4292468..4292818 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag KIM92_RS20810 Protein ID WP_001223208.1
Coordinates 4292812..4293063 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS20785 (4287744) 4287744..4288766 - 1023 WP_001339490.1 ABC transporter permease -
KIM92_RS20790 (4288780) 4288780..4290282 - 1503 WP_021548597.1 sugar ABC transporter ATP-binding protein -
KIM92_RS20795 (4290592) 4290592..4291548 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KIM92_RS20800 (4291858) 4291858..4292388 + 531 WP_000055072.1 inorganic diphosphatase -
KIM92_RS20805 (4292468) 4292468..4292818 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
KIM92_RS20810 (4292812) 4292812..4293063 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KIM92_RS20815 (4293276) 4293276..4293617 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KIM92_RS20820 (4293620) 4293620..4297399 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T202642 WP_000239579.1 NZ_CP074680:c4292818-4292468 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T202642 NZ_CP099543:96771-97238 [Pantoea ananatis]
TTGAAGCTCTATCGCATGACCAAAACGCGCTATCTGGGGTCGGCCTGGAGCGGTTTTGGTGCCCGCGAAGGCGGCGGGCG
CTGGAACAGCGTCGGCGTATCAATGGTTTACGCTTCTGAAACCGCTTCCCTCACCATGCTGGAGACGCTTATCCATCTGC
AAAGCGCCAGCGTGCTGGACTGTTTTACCCTCATGAGCATTGACGTTCCCGACAGGCTGATTGAATGGATTGATATCAAG
CAACTTCCTGACGACTGGGCCGCACCGGAAGCGCCTGCAGCGCTGCGTTTATTTGGTGATGCCTGGATACAAAGCGGGGG
ATCGGTCGCACTGCGCGTGCCCAGCGCCCTTTCGCCCGTAGAATCGAATTATCTTCTCAATCCGGAACATCCCGAATTCA
ATGCTATCGTCAGACAGGCGATAAACATCCCCTTCCTTTTTGACGTACGCTTCTCCCGACAGGCATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT202642 WP_001223208.1 NZ_CP074680:c4293063-4292812 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT202642 NZ_CP099543:96331-96774 [Pantoea ananatis]
ATGAAACAGTTCACTTTACCCGTATCCGCTGCAGAACCGGATCGTCTGTGGCAATTTGCCGGCCTGAGCAGTGCTGACGG
CGTGATGCTGATGGATCAAATCGATGATGGTCTGGATGGCACGGTAGCCAGTCGCATTGTCACCTGGGCGCAAATCTCTC
AGGCAGAACTGCGCCGTATGACCGGCATTGCGGGCTCAACCTTCACCCGCGGCGTTAAGAGCCGCTTCACTGCGGAGCAG
AGCGAACGGCTGGTGCGTTTTATTCGCGTGATGGATCGTGCGGTTGAGCTGTTTGAAGGCGACAAAGAAAAGGCAAGGCA
GTGGCTCAATGAGCCTGCGCGCGCCTTGGGCAATAAAAAACCGGCCGATCTGGTCGCGTCAGAAACCGGGGCTTACGAAG
TGCTGAAACTCATTACCCGGCTTGAACACGGCGTGTATTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References