Detailed information of TA system TA202637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3544791..3545628
• Replicon: chromosome
• Accession: NZ_CP074680
• Organism: Escherichia coli strain MEI008

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KIM92_RS17225
• Protein ID: WP_000227784.1
• Coordinates: 3545086..3545628 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KIM92_RS17220
• Protein ID: WP_001297137.1
• Coordinates: 3544791..3545102 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM92_RS17195 (3539811)	3539811..3540758	+	948	WP_021548571.1	cytochrome o ubiquinol oxidase subunit II	-
KIM92_RS17200 (3540780)	3540780..3542771	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KIM92_RS17205 (3542761)	3542761..3543375	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KIM92_RS17210 (3543375)	3543375..3543704	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KIM92_RS17215 (3543716)	3543716..3544606	+	891	WP_000971336.1	heme o synthase	-
KIM92_RS17220 (3544791)	3544791..3545102	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KIM92_RS17225 (3545086)	3545086..3545628	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KIM92_RS17230 (3545684)	3545684..3546619	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KIM92_RS17235 (3547027)	3547027..3548391	+	1365	WP_001000978.1	MFS transporter	-
KIM92_RS17240 (3548519)	3548519..3549010	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KIM92_RS17245 (3549178)	3549178..3550089	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T202637 WP_000227784.1 NZ_CP074680:3545086-3545628 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T202637 NZ_CP099541:212752-213051 [Pantoea stewartii]
ATGCAATTTGACGTTTACTCACACAAGAATGGTAAAAAATATCCTTATCTTGTTGAAGTACAAAGCTATCTAATAGACAC
ACCAGGCCGTTTAATGGTTGCCCCCCTTGCTCTTCCGGGCGAGTTCGTGGGTTCAGGTGAACTTTATCCTGAGGTACTGG
TTAACGGCAGCAAGTACCGCGTTGTGACAACCGACATTGCATCTGTCCCGGTGAAAGCGTTAGGGGATAAAGTGGGTGAT
GTCAGTCAGTATGAAGCAGCAATAAAAAACGCCATTTTCAGGCTTTTTTGGGGTTTCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT202637 WP_001297137.1 NZ_CP074680:3544791-3545102 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT202637 NZ_CP099541:212531-212749 [Pantoea stewartii]
ATGAAATACAGAACTAACGTCACTATTGATAAAGAAGCCTACGAGATATTTTCATCTGCGGGCTTAAACCTTTCTAAAAT
GGTTAACGAGCTTGTCACTACTGAGGCTCGCAGAATCCGGGCTGAACGCTGGAAAGAAGAAAACCGTGAAAGCATGAAAG
CCTTCTCTGACTTTGTGGGAGAAGAGGGATCGCTTTCTGATATGTACAGGAACTGGTAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3