202636

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3509276..3509894 Replicon chromosome
Accession NZ_CP074680
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KIM92_RS17050 Protein ID WP_001291435.1
Coordinates 3509676..3509894 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KIM92_RS17045 Protein ID WP_000344800.1
Coordinates 3509276..3509650 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS17035 (3504365) 3504365..3505558 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KIM92_RS17040 (3505581) 3505581..3508730 + 3150 WP_255864124.1 efflux RND transporter permease AcrB -
KIM92_RS17045 (3509276) 3509276..3509650 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KIM92_RS17050 (3509676) 3509676..3509894 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KIM92_RS17055 (3510066) 3510066..3510617 + 552 WP_000102568.1 maltose O-acetyltransferase -
KIM92_RS17060 (3510733) 3510733..3511203 + 471 WP_000136192.1 YlaC family protein -
KIM92_RS17065 (3511367) 3511367..3512917 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KIM92_RS17070 (3512959) 3512959..3513312 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KIM92_RS17080 (3513691) 3513691..3514002 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3514103..3515431 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202636 WP_001291435.1 NZ_CP074680:3509676-3509894 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T202636 NZ_CP099540:2369825-2370040 [Pantoea stewartii]
ATGAACAAAACCCTGACTAAAACTGATTATTTGATGCGACTGAGACGTTGTCGCTCAATTGACACGCTCGAAAGAGTGAT
TGAGAAAAATAAGTATGAATTGTCCGATGATGAATTAGCCGTATTTTACTCCGCAGCCGATCACCGCCTGGCTGAACTGA
CGATGAACAAACTGTATGACAAAGTCCCCGTGTCAGTATGGAAATTTGTACGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT202636 WP_000344800.1 NZ_CP074680:3509276-3509650 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT202636 NZ_CP099540:2369423-2369800 [Pantoea stewartii]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACGTGTGTGAGAGTTTGTTTGACGCCAGTATGGC
AACATTAACCGACAGCCATCATGGCTGGGTAAATGACCCTACTGCCGATGGTAATTTACAGCTGAACGATTTGATAGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATTAAGCATGCAGAAGACGAAGCGCTGATTACCCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTTAGCAACTATAGCGTTAACACTCAGGATCTGCAGCGCTGGCAACGTTCAGCAAAACGTTT
ATTTAATCTGTTTCAAGAAGAGTGCGCTTATCTTCAACAGCCAAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References